Linked Life Data

15255077

Source:http://linkedlifedata.com/resource/pubmed/id/15255077

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-7-16
pubmed:abstractText
Because of the increasing interest on stimulating fracture healing, knowledge about the role and chronology of growth factors during the healing process is important. The purpose of this study was to quantify the protein concentration of IGF-I and TGF-beta1 during rat tibial fracture healing 5, 10, and 15 days after fracture using ELISA methods and to analyze the distribution of the proteins and the related mRNA expression in the fracture callus by immunohistochemistry and in situ hybridization. The following three groups were analyzed: Fractured tibiae intramedullary stabilized with K-wires coated with IGF-I and TGF-beta1 compared with fractures stabilized with uncoated K-wires and unfractured tibiae. The weight of the callus increased during the healing process in both experimental groups. The protein concentration of IGF-I and TGF-beta1 in the fracture callus showed significant changes between the investigated time points and treatment groups compared with the unfractured tibia. IGF-I increased with healing time whereas TGF-beta1 revealed a constantly elevated level at the investigated time points. Mesenchymal cells, osteoblasts, osteocytes, proliferating and immature chondrocytes, and osteoclasts expressed both growth factors. No differences in the expression and localization pattern of the growth factors were detectable among the groups. Using the different methods for quantification and visualization of the growth factors, no differences (except the increased IGF-I concentration at day 15 in the growth factor group) were seen between the normal and the growth factor-stimulated fracture healing as an indication for physiological healing after exogenous growth factor treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0171-967X
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
388-97
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15255077-Absorbable Implants, pubmed-meshheading:15255077-Animals, pubmed-meshheading:15255077-Disease Models, Animal, pubmed-meshheading:15255077-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:15255077-Female, pubmed-meshheading:15255077-Fracture Healing, pubmed-meshheading:15255077-Humans, pubmed-meshheading:15255077-Immunoenzyme Techniques, pubmed-meshheading:15255077-In Situ Hybridization, pubmed-meshheading:15255077-Insulin-Like Growth Factor I, pubmed-meshheading:15255077-Polyesters, pubmed-meshheading:15255077-Polymers, pubmed-meshheading:15255077-Proteins, pubmed-meshheading:15255077-RNA, Messenger, pubmed-meshheading:15255077-Rats, pubmed-meshheading:15255077-Rats, Sprague-Dawley, pubmed-meshheading:15255077-Tibial Fractures, pubmed-meshheading:15255077-Transforming Growth Factor beta, pubmed-meshheading:15255077-Transforming Growth Factor beta1
pubmed:year
2004
pubmed:articleTitle
Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing.
pubmed:affiliation
Department of Trauma and Reconstructive Surgery, Charité, Humboldt-University of Berlin, Berlin, Germany. britt.wildemann@charite.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't