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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-8-2
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pubmed:abstractText |
We previously reported that five repeated pulses of 5-HT lead to down-regulation of the TM-apCAM isoform at the surface of Aplysia sensory neurons (SNs). We here examined whether apCAM down-regulation is required for 5-HT-induced long-term facilitation. We also analyzed the role of the cytoplasmic and extracellular domains by overexpressing various apCAM mutants by DNA microinjection. When TM-apCAM was up-regulated in SNs by DNA microinjection, five pulses of 5-HT failed to produce either synaptic facilitation or an enhancement of synaptic growth, suggesting that down-regulation of apCAM is required for 5-HT-induced EPSP enhancement and new varicosity formation. However, disrupting the extracellular domain function of overexpressed apCAM with a specific antibody restored 5-HT-induced excitatory postsynaptic potential increase but not synaptic growth. The overexpression of the MAP Kinase mutant of TM-apCAM, which is not internalized by 5-HT, inhibited new varicosity formation, but did not inhibit excitatory postsynaptic potential increase. Deletion mutants containing only the cytoplasmic portion of apCAM blocked 5-HT-induced synaptic growth but not excitatory postsynaptic potential increase. Thus, our data suggest that TM-apCAM may act as a suppressor of both synaptic-strength enhancement in pre-existing synapses and of new synaptic varicosity formation in the nonsynaptic region, via different mechanisms.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-10414963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-10535740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-10608864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-10798405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-11520928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-11533225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-11738028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-12082080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-12202822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-12360325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-1310540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-14527440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-1585176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-1585177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-1673849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-1680564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-1698945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-2277077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-2389145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-2559759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-2592997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-2642005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-2921606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-3353385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-3775383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-3958793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-6655493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-6828885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-7669290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-7790898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-7790903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-7907362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-8137425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-8384857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-8466181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-9014173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-9020359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-9208859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-9264029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-9592080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15254221-9766387
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1072-0502
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
421-35
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15254221-Animals,
pubmed-meshheading:15254221-Aplysia,
pubmed-meshheading:15254221-Cell Adhesion Molecules,
pubmed-meshheading:15254221-DNA,
pubmed-meshheading:15254221-Electrophysiology,
pubmed-meshheading:15254221-Excitatory Postsynaptic Potentials,
pubmed-meshheading:15254221-Ganglia, Invertebrate,
pubmed-meshheading:15254221-Gene Deletion,
pubmed-meshheading:15254221-Gene Expression Regulation,
pubmed-meshheading:15254221-Long-Term Potentiation,
pubmed-meshheading:15254221-Microinjections,
pubmed-meshheading:15254221-Neurons, Afferent,
pubmed-meshheading:15254221-Protein Structure, Tertiary,
pubmed-meshheading:15254221-Serotonin,
pubmed-meshheading:15254221-Signal Transduction,
pubmed-meshheading:15254221-Structure-Activity Relationship,
pubmed-meshheading:15254221-Synapses,
pubmed-meshheading:15254221-Synaptic Transmission
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pubmed:articleTitle |
Role of Aplysia cell adhesion molecules during 5-HT-induced long-term functional and structural changes.
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pubmed:affiliation |
National Research Laboratory of Neurobiology, Institute of Molecular Biology and Genetics, School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 151-742, Korea.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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