| pubmed-article:15251961 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15251961 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:15251961 | lifeskim:mentions | umls-concept:C0684249 | lld:lifeskim |
| pubmed-article:15251961 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:15251961 | lifeskim:mentions | umls-concept:C0006279 | lld:lifeskim |
| pubmed-article:15251961 | lifeskim:mentions | umls-concept:C1550101 | lld:lifeskim |
| pubmed-article:15251961 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:15251961 | pubmed:dateCreated | 2004-7-14 | lld:pubmed |
| pubmed-article:15251961 | pubmed:abstractText | Recently, it was shown that it is possible to isolate free circulating DNA from plasma/serum of patients with benign and malignant diseases. In addition, several groups were able to detect tumor-associated alterations in these nucleic acids. We wondered whether any nucleic acids are detectable in cell-free bronchial lavage supernatants, which until now have been discarded after cell harvest. Additionally, we wanted to find out if it is possible to detect tumor-associated alterations in these DNA molecules. DNA was isolated from cell-free lavage supernatants from 30 lung cancer patients, and the DNA was examined for microsatellite alterations. Intact DNA could be isolated from all cell-free bronchial lavage supernatants. Microsatellite alterations were found in lavage supernatants of 12 of 30 patients and in lavage cells of 6 of 30 patients. Altogether, alterations were found in 14 of 30 patients. Thus, we could demonstrate for the first time that it is possible to isolate intact DNA from cell-free bronchial lavage supernatants. Their quantity and quality are sufficient for further amplification via polymerase chain reaction. Altogether, tumor-associated changes were detected in the DNA of 47% of the patients that were analyzed. | lld:pubmed |
| pubmed-article:15251961 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15251961 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15251961 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15251961 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15251961 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15251961 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15251961 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15251961 | pubmed:issn | 0077-8923 | lld:pubmed |
| pubmed-article:15251961 | pubmed:author | pubmed-author:WittChristian... | lld:pubmed |
| pubmed-article:15251961 | pubmed:author | pubmed-author:SchmidtBerndB | lld:pubmed |
| pubmed-article:15251961 | pubmed:author | pubmed-author:JandrigBurkha... | lld:pubmed |
| pubmed-article:15251961 | pubmed:author | pubmed-author:Fleischhacker... | lld:pubmed |
| pubmed-article:15251961 | pubmed:author | pubmed-author:CarstensenTim... | lld:pubmed |
| pubmed-article:15251961 | pubmed:author | pubmed-author:EngelEileenE | lld:pubmed |
| pubmed-article:15251961 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15251961 | pubmed:volume | 1022 | lld:pubmed |
| pubmed-article:15251961 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15251961 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15251961 | pubmed:pagination | 202-10 | lld:pubmed |
| pubmed-article:15251961 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:15251961 | pubmed:meshHeading | pubmed-meshheading:15251961... | lld:pubmed |
| pubmed-article:15251961 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15251961 | pubmed:articleTitle | Detection of cell-free DNA in bronchial lavage fluid supernatants of patients with lung cancer. | lld:pubmed |
| pubmed-article:15251961 | pubmed:affiliation | Charité-Universitätsmedizin Berlin, Medizinische Klinik II, Pulmologie, Berlin, Germany. | lld:pubmed |
| pubmed-article:15251961 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15251961 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15251961 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
