Linked Life Data

15249551

Source:http://linkedlifedata.com/resource/pubmed/id/15249551

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-8-27
pubmed:abstractText
Increased homocysteine is associated with the pregnancy complication preeclampsia and with later-life cardiovascular disease. Although elevated homocysteine persists after pregnancy, the vascular changes of preeclampsia abate with delivery, and cardiovascular disease occurs decades later. This suggests the vasculature during pregnancy may manifest increased sensitivity to homocysteine. We used the cystathionine-beta synthase (CBS)-deficient transgenic mouse to investigate whether hyperhomocysteinemia would differentially affect vascular function in nonpregnant and pregnant animals. Mesenteric arteries from nonpregnant and midpregnant (14 to 16 days) wild-type, heterozygous, and homozygous CBS-deficient transgenic mice were investigated for their response to vasoconstriction, endothelial-dependent, and endothelial-independent relaxation using an isometric wire myograph system. Endothelial-dependent vasodilation was similar in arteries from nonpregnant heterozygous and wild-type mice. In contrast, endothelial-dependent relaxation was reduced significantly in arteries from pregnant heterozygous animals compared with wild-type mice. Inhibition of NO synthesis blunted relaxation in arteries from pregnant wild-type but not pregnant heterozygous mice. Endothelial-dependent relaxation was restored by in vitro pretreatment with the tetrahydrobiopterin precursor sepiapterin. These data indicate that in pregnant mice, endothelial-dependent vasodilation is more sensitive to the effect of increased homocysteine than arteries from nonpregnant mice. This effect appears to result from a loss in NO-mediated relaxation that may be mediated by the oxidative inactivation of the NO synthase cofactor tetrahydrobiopterin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1524-4563
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-33
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15249551-Animals, pubmed-meshheading:15249551-Cystathionine beta-Synthase, pubmed-meshheading:15249551-Endothelium, Vascular, pubmed-meshheading:15249551-Female, pubmed-meshheading:15249551-Hyperhomocysteinemia, pubmed-meshheading:15249551-Mesenteric Arteries, pubmed-meshheading:15249551-Mice, pubmed-meshheading:15249551-Mice, Knockout, pubmed-meshheading:15249551-Mice, Transgenic, pubmed-meshheading:15249551-Nitric Oxide Synthase, pubmed-meshheading:15249551-Nitric Oxide Synthase Type II, pubmed-meshheading:15249551-Nitric Oxide Synthase Type III, pubmed-meshheading:15249551-Oxidative Stress, pubmed-meshheading:15249551-Phenylephrine, pubmed-meshheading:15249551-Pregnancy, pubmed-meshheading:15249551-Pregnancy Complications, pubmed-meshheading:15249551-Pterins, pubmed-meshheading:15249551-Tyrosine, pubmed-meshheading:15249551-Vasodilation
pubmed:year
2004
pubmed:articleTitle
Moderate hyperhomocysteinemia decreases endothelial-dependent vasorelaxation in pregnant but not nonpregnant mice.
pubmed:affiliation
Magee-Women's Research Institute and Department of Obstetrics and Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213. rsirwp@mwri.magee.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.