Linked Life Data

1524792

Source:http://linkedlifedata.com/resource/pubmed/id/1524792

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1992-10-22
pubmed:abstractText
This report describes experiments to detect oligonucleotide hybridization at the surface of a fibre-optic evanescent wave biosensor. Conditions were optimized and the time course of hybridization reactions were found to be very rapid compared to conventional hybridization assays. Binding was easily reversed by heating and the sensor surface could be reused many times. Short (16- and 20-mer) oligonucleotides bound to the waveguide surface could be used to detect fluorescein-labelled complementary sequences at the nanomolar level. Polymerase chain reaction was used to generate a 204-base oligomer that was attached to the waveguide surface. Fluorescein-labelled oligos bound at either the proximal or distal ends of this probe were found to give similar outputs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0956-5663
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
487-93
pubmed:dateRevised
2009-7-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Gene probe assays on a fibre-optic evanescent wave biosensor.
pubmed:affiliation
Chemical and Biological Defence Establishment, Porton Down, Wiltshire, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't