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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2004-7-12
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pubmed:abstractText |
DNA strand breaks containing 3'-phosphoglycolate (3'-PG) ends are the major lesions induced by ionizing radiation. The repair of this lesion is not completely understood and several activities are thought to be involved in processing of 3'-PG ends. In this study we examined activities in human whole cell extracts (WCE) responsible for removal of 3'-PG. Using a radiolabelled oligonucleotide containing a single nucleotide gap with internal 5'-phosphate and 3'-PG ends, we demonstrate that the major 3'-PG activity in human WCE is Mg2+ dependent and that this activity co-purifies with AP endonuclease 1 (APE1) over phosphocellulose and gel filtration chromatography. Furthermore, immunodepletion of APE1 from active gel filtration fractions using APE1 specific antibodies reveals that the major activity against 3'-PG in human WCE is APE1.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-10325434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-10460157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-10874010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-11601988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-12023295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-12483509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-1375993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-1380322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-1719477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-1719484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-1722334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-6193397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-6259165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-7516064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-7979257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-8469282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-8876170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-8995436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-9171104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-9235895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-9786884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15247342-9952316
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3531-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2004
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pubmed:articleTitle |
APE1 is the major 3'-phosphoglycolate activity in human cell extracts.
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pubmed:affiliation |
MRC Radiation and Genome Stability Unit, Harwell, Oxfordshire OX11 0RD, UK.
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pubmed:publicationType |
Journal Article
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