Linked Life Data

15246698

Source:http://linkedlifedata.com/resource/pubmed/id/15246698

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2004-7-12
pubmed:abstractText
Corticotropin-releasing factor (CRF), in addition to its role as a hormone in the stress response, functions as a neuromodulator in the cerebellum, where it enhances both the spontaneous and amino acid induced firing rate of Purkinje cells. In the cerebellum, CRF and its two types of receptors (CRF-R(1) and CRF-R(2)) are present during cerebellar development at ages that precede the onset of afferent ingrowth and synaptogenesis, suggesting a distinct role during early cerebellar development. The present study was undertaken to determine whether CRF enhances the survival of cerebellar neurons, in particular GABAergic neurons. Primary cultures of cerebellar neurons obtained from embryonic day 18 mice were composed primarily, but not exclusively, of GABAergic neurons. Although CRF-R(1) is present in most neurons in this culture system, when CRF was added to the medium, no significant change in neuronal survival was observed when compared to control cultures. It is possible that a role for CRF is not seen in growth-promoting culture medium at the plating density chosen for this study and may only be evident when the cells have been exposed to conditions that reduce the likelihood of survival, such as exposure to neurotoxins such as AraC. We propose that, because AraC increases the number of cleaved caspase-3 positive cells, indicating apoptosis, it is possible that a CRF effect involves an inhibition of the apoptotic pathway. Cultures treated with AraC had a decrease in the total number of GABAergic neurons and an increase in apoptotic cells as measured with the apoptotic marker cleaved caspase-3. Co-treatment with CRF rescued many GABAergic neurons. It is interesting to note that apoptotic cells do not exhibit GABA or c-fos positive immunolabeling. Thus, these data support the concept that CRF plays a neuroprotective role in the survival of GABAergic cerebellar neurons in culture after exposure to a neurotoxin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0165-3806
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
151
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-28
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15246698-Animals, pubmed-meshheading:15246698-Cell Survival, pubmed-meshheading:15246698-Cells, Cultured, pubmed-meshheading:15246698-Cerebellum, pubmed-meshheading:15246698-Corticotropin-Releasing Hormone, pubmed-meshheading:15246698-Cytarabine, pubmed-meshheading:15246698-Dose-Response Relationship, Drug, pubmed-meshheading:15246698-Drug Interactions, pubmed-meshheading:15246698-Embryo, Mammalian, pubmed-meshheading:15246698-Female, pubmed-meshheading:15246698-Immunohistochemistry, pubmed-meshheading:15246698-Male, pubmed-meshheading:15246698-Mice, pubmed-meshheading:15246698-Mice, Inbred C57BL, pubmed-meshheading:15246698-Microscopy, Confocal, pubmed-meshheading:15246698-Neurons, pubmed-meshheading:15246698-Neurotoxins, pubmed-meshheading:15246698-Pregnancy, pubmed-meshheading:15246698-Receptors, Corticotropin-Releasing Hormone, pubmed-meshheading:15246698-gamma-Aminobutyric Acid
pubmed:year
2004
pubmed:articleTitle
Corticotropin releasing factor enhances survival of cultured GABAergic cerebellar neurons after exposure to a neurotoxin.
pubmed:affiliation
Department of Neuroscience, The Ohio State University, Columbus, OH 43210, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.