Source:http://linkedlifedata.com/resource/pubmed/id/15245866
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2004-7-12
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pubmed:abstractText |
Bradykinin potentiating peptides (BPPs) from Bothrops jararaca venom were described in the middle of 1960s and were the first natural inhibitors of the angiotensin-converting enzyme displaying strong anti-hypertensive effects in human subjects. The BPPs can be recognized by their typical pyroglutamyl proline-rich oligopeptide sequences presenting invariably a proline residue at the C-terminus. In the present study, we identified 18 BPPs, most of them already described for the B. jararaca venom. We isolated and sequenced new peptides ranging from 5 to 14 amino acid residues exhibiting similar amino acid sequence features. The applied methodology consisted of a strait two-step liquid chromatography, followed by mass spectrometry analysis. Besides the amino acid sequence homology, the corresponding synthetic peptides were able to potentiate bradykinin on the isolated guinea-pig ileum.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0196-9781
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1085-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15245866-Angiotensin-Converting Enzyme Inhibitors,
pubmed-meshheading:15245866-Animals,
pubmed-meshheading:15245866-Bothrops,
pubmed-meshheading:15245866-Chromatography, Gel,
pubmed-meshheading:15245866-Chromatography, Liquid,
pubmed-meshheading:15245866-Oligopeptides,
pubmed-meshheading:15245866-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:15245866-Viper Venoms
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pubmed:year |
2004
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pubmed:articleTitle |
Identification of five new bradykinin potentiating peptides (BPPs) from Bothrops jararaca crude venom by using electrospray ionization tandem mass spectrometry after a two-step liquid chromatography.
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pubmed:affiliation |
Center for Applied Toxinology CAT-CEPID, Instituto Butantan, Av. Vital Brasil, 1500, Sao Paulo, SP 05503-900.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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