Linked Life Data

15243721

Source:http://linkedlifedata.com/resource/pubmed/id/15243721

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-2-3
pubmed:abstractText
We characterized the effects of intracellular Mg(2+) (Mg(2+) (i)) on potassium currents mediated by the Kv1.5 and Kv2.1 channels expressed in Xenopus oocytes. Increase in Mg(2+) (i) caused a voltage-dependent block of the current amplitude, apparent acceleration of the current kinetics (explained by a corresponding shift in the steady-state activation) and leftward shifts in activation and inactivation dependencies for both channels. The voltage-dependent block was more potent for Kv2.1 [dissociation constant at 0 mV, K(d)(0), was approximately 70 mM and the electric distance of the Mg(2+) binding site, delta, was 0.2] than for the Kv1.5 channel [K(d)(0) approximately 40 mM and delta = 0.1]. Similar shifts in the voltage-dependent parameters for both channels were described by the Gouy-Chapman formalism with the negative charge density of 1 e(-)/100 A(2). Additionally, Mg(2+) (i) selectively reduced a non-inactivating current and increased the accumulation of inactivation of the Kv1.5, but not the Kv2.1 channel. A potential functional role of the differential effects of Mg(2+) (i) on the Kv channels is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0175-7571
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
42-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Effects of intracellular magnesium on Kv1.5 and Kv2.1 potassium channels.
pubmed:affiliation
Department of Pharmacy and Pharmacology, University of Bath, Bath , BA2 7AY, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't