Source:http://linkedlifedata.com/resource/pubmed/id/15241500
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2004-9-14
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| pubmed:abstractText |
S100 proteins belong to the EF-hand Ca2+-binding protein family and are involved in the regulation of a variety of cellular processes. Individual S100 proteins are expressed in cell- and tissue-specific manners, and functional deterioration of S100 proteins leads to a number of human diseases, including cancer. We previously demonstrated that S100C/A11 was translocated to nuclei and inhibited DNA synthesis in human keratinocytes when exposed to high Ca2+. In the present study we examined the effects of synthetic partial peptides of S100C/A11 on human carcinoma cell lines. Only an N-terminal peptide with 19 amino acid residues (MAK19) showed cytotoxicity to the cell lines in dose- and time-dependent manners when introduced into cells by flanking the HIV-TAT protein transduction domain (TAT-MAK19). Pulse field electrophoresis revealed that DNA of the treated cells was partially degradated. Annexin V, a marker of cellular apoptosis, was detected in the cells treated with TAT-MAK19 by immunostaining and flow cytometry. The induction of apoptotic cell death was apparently independent of p53, p21WAF1/CIP1, and caspase activity, but treatment with TAT-MAK19 resulted in partial translocation of apoptosis-inducing factor (AIF) from the cytoplasm to nuclei. These results indicate that MAK19 induces apoptosis in human cell lines and may therefore lead to the establishment of a new molecular target for the treatment of human cancer.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/S100A11 protein, human
|
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
|
| pubmed:issn |
0946-2716
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
82
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
612-20
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| pubmed:dateRevised |
2011-7-8
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| pubmed:meshHeading |
pubmed-meshheading:15241500-Amino Acid Sequence,
pubmed-meshheading:15241500-Apoptosis,
pubmed-meshheading:15241500-Carcinoma,
pubmed-meshheading:15241500-Carcinoma, Squamous Cell,
pubmed-meshheading:15241500-Cell Cycle Proteins,
pubmed-meshheading:15241500-Cell Line, Transformed,
pubmed-meshheading:15241500-Cell Line, Tumor,
pubmed-meshheading:15241500-Cells, Cultured,
pubmed-meshheading:15241500-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:15241500-DNA Replication,
pubmed-meshheading:15241500-Drug Design,
pubmed-meshheading:15241500-Gene Expression Regulation,
pubmed-meshheading:15241500-Gene Products, tat,
pubmed-meshheading:15241500-Humans,
pubmed-meshheading:15241500-Melanoma,
pubmed-meshheading:15241500-Molecular Sequence Data,
pubmed-meshheading:15241500-Peptide Fragments,
pubmed-meshheading:15241500-Protein Structure, Tertiary,
pubmed-meshheading:15241500-Protein Transport,
pubmed-meshheading:15241500-S100 Proteins
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| pubmed:year |
2004
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| pubmed:articleTitle |
Introduction of an N-terminal peptide of S100C/A11 into human cells induces apoptotic cell death.
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| pubmed:affiliation |
Department of Dermatology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikatachou, 700-8558, Okayama, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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