Source:http://linkedlifedata.com/resource/pubmed/id/15238077
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2004-7-7
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| pubmed:abstractText |
A replication-defective adenovirus-LacZ recombinant virus (AdLacZ) was injected intravenously into IRF-1(-/-) mice and wild-type mice to characterize the contribution of IRF-1 to the immune-mediated clearance of Ad vector. Compared with wild-type mice, IRF-1(-/-) mice expressed higher levels of the LacZ gene product in the liver. After infusion of the AdLacZ, the expression of IRF-1 mRNA was upregulated in the liver of wild-type mice, but not in IRF-1(-/-) mice. Both spleen and liver mononuclear cells from IRF-1(-/-) mice initially exhibited a markedly lower number of NK, NK-T and CD8 T cells. At day 7 after the administration of AdLacZ, there was a significantly increased population of NK, NK-T and CD8 T cells in both spleen and liver, and also CD11b(+) cells in liver of IRF-1(-/-) mice, compared with the increased in wild-type mice. As IRF-1 is an important signal for production of IFN-gamma by CD8 T and NK cells as well as production of IL-12 by CD11b(+) cells, we determined whether there were lower levels of these cytokines in IRF-1(-/-) mice after Ad challenge. Surprisingly, there were lower levels of IL-12, but higher levels of IFN-gamma and IL-18 in IRF-1(-/-) compared with wild-type mice at day 7 after administration with AdLacZ. These results indicate that delayed clearance of Ad is associated with partial correction of defects of the NK, NK-T and CD8 T cells and increased production of IFN-gamma and IL-18 in IRF-1(-/-) mice.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Irf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0300-9475
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
60
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
89-99
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15238077-Adenoviridae,
pubmed-meshheading:15238077-Adenoviridae Infections,
pubmed-meshheading:15238077-Animals,
pubmed-meshheading:15238077-Antibodies, Viral,
pubmed-meshheading:15238077-Antigens, Viral,
pubmed-meshheading:15238077-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15238077-Cytokines,
pubmed-meshheading:15238077-DNA-Binding Proteins,
pubmed-meshheading:15238077-Female,
pubmed-meshheading:15238077-Genetic Vectors,
pubmed-meshheading:15238077-Interferon Regulatory Factor-1,
pubmed-meshheading:15238077-Killer Cells, Natural,
pubmed-meshheading:15238077-Liver,
pubmed-meshheading:15238077-Macrophages,
pubmed-meshheading:15238077-Male,
pubmed-meshheading:15238077-Mice,
pubmed-meshheading:15238077-Mice, Inbred C57BL,
pubmed-meshheading:15238077-Mice, Knockout,
pubmed-meshheading:15238077-Phosphoproteins,
pubmed-meshheading:15238077-RNA,
pubmed-meshheading:15238077-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15238077-Spleen
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| pubmed:articleTitle |
Defective clearance of adenovirus in IRF-1 mice associated with defects in NK and T cells but not macrophages.
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| pubmed:affiliation |
Department of Medicine, The University of Alabama at Birmingham, 701 S. 19th Street, Birmingham, AL 35294-0007, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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