Linked Life Data

15236231

Source:http://linkedlifedata.com/resource/pubmed/id/15236231

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-7-5
pubmed:abstractText
The number of cerebellar Purkinje cells is increased by over 40% in young transgenic mice that overexpress a human Bcl-2 transgene (Hu-Bcl-2). To determine whether the Bcl-2-mediated rescue of Purkinje cells persists through life, the numbers of Purkinje cells were estimated in 6-, 12-, 18-, and 24-month-old Hu-Bcl-2 transgenic mice and age-matched controls. In addition, the expression of four markers for Purkinje cell differentiation, calbindin (CaBP), the 67-kDa isoform of glutamic acid decarboxylase (GAD67), gamma-aminobutyric acid transaminase (GABA-T), and the NMDA-R1 receptor subtype (NMDA-NR1) was analyzed in 6-month-old Hu-Bcl-2 transgenics and controls to determine whether overexpression of Bcl-2 and rescue from naturally occurring cell death affects the normal differentiation of Purkinje cells. The estimates of Purkinje cell numbers showed that the number of Purkinje cells in the Hu-Bcl-2 transgenics declines after 6 months to approach wild-type values by 18 months. Although the exogenous human BCL-2 is still expressed in Purkinje cells at 24 months, the expression levels of human BCL-2 appear to decline significantly after 6 months, suggesting that survival of the supernumary Purkinje cells depends on the sustained overexpression of Bcl-2. All the Purkinje cells in the Hu-Bcl-2 transgenic mice appeared to express normal levels of the differentiation markers analyzed so there was no evidence for a class of Purkinje cells that do not differentiate normally when rescued from naturally occurring cell death.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9967
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
475
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
481-92
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15236231-4-Aminobutyrate Transaminase, pubmed-meshheading:15236231-Aging, pubmed-meshheading:15236231-Animals, pubmed-meshheading:15236231-Apoptosis, pubmed-meshheading:15236231-Blotting, Western, pubmed-meshheading:15236231-Calcium-Binding Protein, Vitamin D-Dependent, pubmed-meshheading:15236231-Cell Count, pubmed-meshheading:15236231-Cell Differentiation, pubmed-meshheading:15236231-Glutamate Decarboxylase, pubmed-meshheading:15236231-Immunohistochemistry, pubmed-meshheading:15236231-In Situ Hybridization, pubmed-meshheading:15236231-Isoenzymes, pubmed-meshheading:15236231-Mice, pubmed-meshheading:15236231-Mice, Transgenic, pubmed-meshheading:15236231-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:15236231-Purkinje Cells, pubmed-meshheading:15236231-Receptors, N-Methyl-D-Aspartate
pubmed:year
2004
pubmed:articleTitle
Cerebellar Purkinje cell loss in aging Hu-Bcl-2 transgenic mice.
pubmed:affiliation
Lab. Développement et Vieillissement du Système Nerveux (DVSN), UMR NPA 7102 Centre National de la Recherche Scientifique and Université Pierre and Marie Curie, 75005 Paris, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.