Linked Life Data

15231568

Source:http://linkedlifedata.com/resource/pubmed/id/15231568

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2004-10-6
pubmed:abstractText
During ontogenesis and the entire adult life hematopoietic stem and progenitor cells have the capability to migrate. In comparison to the process of peripheral leukocyte migration in inflammatory responses, the molecular and cellular mechanisms governing the migration of these cells remain poorly understood. A common feature of migrating cells is that they need to become polarized before they migrate. Here we have investigated the issue of cell polarity of hematopoietic stem/progenitor cells in detail. We found that human CD34(+) hematopoietic cells (1) acquire a polarized cell shape upon cultivation, with the formation of a leading edge at the front pole and a uropod at the rear pole; (2) exhibit an amoeboid movement, which is similar to the one described for migrating peripheral leukocytes; and (3) redistribute several lipid raft markers including cholesterol-binding protein prominin-1 (CD133) in specialized plasma membrane domains. Furthermore, polarization of CD34(+) cells is stimulated by early acting cytokines and requires the activity of phosphoinositol-3-kinase as previously reported for peripheral leukocyte polarization. Together, our data reveal a strong correlation between polarization and migration of peripheral leukocytes and hematopoietic stem/progenitor cells and suggest that they are governed by similar mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2332-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15231568-Antigens, CD, pubmed-meshheading:15231568-Antigens, CD34, pubmed-meshheading:15231568-Antigens, CD44, pubmed-meshheading:15231568-Biological Markers, pubmed-meshheading:15231568-Cell Movement, pubmed-meshheading:15231568-Cell Polarity, pubmed-meshheading:15231568-Cell Size, pubmed-meshheading:15231568-Cells, Cultured, pubmed-meshheading:15231568-Gene Expression, pubmed-meshheading:15231568-Glycoproteins, pubmed-meshheading:15231568-Hematopoietic Stem Cells, pubmed-meshheading:15231568-Humans, pubmed-meshheading:15231568-Membrane Lipids, pubmed-meshheading:15231568-Membrane Microdomains, pubmed-meshheading:15231568-Peptides, pubmed-meshheading:15231568-Phosphatidylinositol 3-Kinases, pubmed-meshheading:15231568-Recombinant Fusion Proteins, pubmed-meshheading:15231568-Transfection
pubmed:year
2004
pubmed:articleTitle
Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells.
pubmed:affiliation
Institut für Transplantationsdiagnostik and Zelltherapeutika, Heinrich Heine Universität Düsseldorf, Moorenstr 5, Geb 14.80, D-40225 Düsseldorf, Germany. giebel@itz.uni-duesseldorf.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't