Linked Life Data

15229132

Source:http://linkedlifedata.com/resource/pubmed/id/15229132

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-10-21
pubmed:abstractText
H1t is a novel linker histone variant synthesized in mid- to late pachytene spermatocytes. Its regulatory region is of interest because developmentally specific expression has been impressed on an otherwise ubiquitously expressed promoter. Using competitive band-shift assays and specific antisera, we have now shown that the H1t-60 CCTAGG palindrome motif region binds members of the RFX family of transcriptional regulators. The testis-specific binding complex contains RFX2, probably as a homodimer. Other DNA-protein complexes obtained from testis as well as somatic organs contain RFX1, primarily as a heterodimer. Western blots confirmed that RFX2 expression is greatly enhanced in adult testis and that RFX2 is equally prominent in highly enriched populations of late pachytene spermatocytes and round spermatids. Immunohistochemistry carried out on mouse testis showed that RFX2 is strongly expressed in pachytene spermatocytes, remains high in early round spermatids, and declines only in advance of nuclear condensation. Maximum expression correlates well with the appearance of H1t. In contrast, RFX1 immunoreactivity in germ cells was only detected in late round spermatids. RFX-specific band complexes were also identified for both the mouse lamin C2 and Sgy promoters, using either testis nuclear extracts or in vitro-synthesized RFX2. These results call attention to RFX2 as a transcription factor with obvious potential for the regulation of gene expression during meiosis and the early development of spermatids.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1551-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15229132-Amino Acid Motifs, pubmed-meshheading:15229132-Animals, pubmed-meshheading:15229132-Base Sequence, pubmed-meshheading:15229132-Binding Sites, pubmed-meshheading:15229132-Blotting, Western, pubmed-meshheading:15229132-Cell Nucleus, pubmed-meshheading:15229132-DNA, pubmed-meshheading:15229132-DNA-Binding Proteins, pubmed-meshheading:15229132-Histones, pubmed-meshheading:15229132-Immunohistochemistry, pubmed-meshheading:15229132-Laminin, pubmed-meshheading:15229132-Male, pubmed-meshheading:15229132-Meiosis, pubmed-meshheading:15229132-Mice, pubmed-meshheading:15229132-Nuclear Proteins, pubmed-meshheading:15229132-Pachytene Stage, pubmed-meshheading:15229132-Promoter Regions, Genetic, pubmed-meshheading:15229132-RNA-Binding Proteins, pubmed-meshheading:15229132-Rats, pubmed-meshheading:15229132-Rats, Sprague-Dawley, pubmed-meshheading:15229132-Spermatids, pubmed-meshheading:15229132-Spermatocytes, pubmed-meshheading:15229132-Spermatogenesis, pubmed-meshheading:15229132-Spermatozoa, pubmed-meshheading:15229132-Testis, pubmed-meshheading:15229132-Tissue Distribution, pubmed-meshheading:15229132-Transcription, Genetic, pubmed-meshheading:15229132-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
RFX2 is a potential transcriptional regulatory factor for histone H1t and other genes expressed during the meiotic phase of spermatogenesis.
pubmed:affiliation
Department of Chemistry and Biochemistry and The School of Medicine, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.