Source:http://linkedlifedata.com/resource/pubmed/id/15225727
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
15
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pubmed:dateCreated |
2004-6-30
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pubmed:abstractText |
High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0960-894X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4065-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15225727-Animals,
pubmed-meshheading:15225727-Antibodies, Catalytic,
pubmed-meshheading:15225727-Flow Cytometry,
pubmed-meshheading:15225727-Fructose-Bisphosphate Aldolase,
pubmed-meshheading:15225727-Hybridomas,
pubmed-meshheading:15225727-Kinetics,
pubmed-meshheading:15225727-Recombinant Proteins,
pubmed-meshheading:15225727-Transfection
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pubmed:year |
2004
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pubmed:articleTitle |
Flow cytometric screening of aldolase catalytic antibodies.
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pubmed:affiliation |
The Skaggs Institute for Chemical Biology and Departments of Molecular Biology and Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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