Linked Life Data

15225553

Source:http://linkedlifedata.com/resource/pubmed/id/15225553

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-6-30
pubmed:abstractText
Selective activation of Rac GTPase signaling pathways requires the specific release of Rac from RhoGDI complexes. We identified a RhoGDI kinase from bovine brain as p21-activated kinase (Pak). Pak1 binds and phosphorylates RhoGDI both in vitro and in vivo at Ser101 and Ser174. This resulted in dissociation of Rac1-RhoGDI, but not RhoA-RhoGDI, complexes, as determined by in vitro assays of complexation and in vivo by coimmunoprecipitation analysis. We observed that Cdc42-induced Rac1 activation is inhibited by expression of Pak1 autoinhibitory domain. The dissociation of Rac1 from RhoGDI and its subsequent activation stimulated by PDGF or EGF is also attenuated by Pak1 autoinhibitory domain, and this is dependent on the ability of RhoGDI to be phosphorylated at Ser101/174. These results support a role for Pak1-mediated RhoGDI phosphorylation as a mechanism for Cdc42-mediated Rac activation, and suggest the possibility of Rac-induced positive feed-forward regulation of Rac activity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1097-2765
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-27
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15225553-Animals, pubmed-meshheading:15225553-Binding Sites, pubmed-meshheading:15225553-Cattle, pubmed-meshheading:15225553-Epidermal Growth Factor, pubmed-meshheading:15225553-Guanine Nucleotide Dissociation Inhibitors, pubmed-meshheading:15225553-HeLa Cells, pubmed-meshheading:15225553-Humans, pubmed-meshheading:15225553-Phosphorylation, pubmed-meshheading:15225553-Platelet-Derived Growth Factor, pubmed-meshheading:15225553-Protein Structure, Tertiary, pubmed-meshheading:15225553-Protein-Serine-Threonine Kinases, pubmed-meshheading:15225553-Signal Transduction, pubmed-meshheading:15225553-cdc42 GTP-Binding Protein, pubmed-meshheading:15225553-p21-Activated Kinases, pubmed-meshheading:15225553-rac GTP-Binding Proteins, pubmed-meshheading:15225553-rac1 GTP-Binding Protein
pubmed:year
2004
pubmed:articleTitle
Phosphorylation of RhoGDI by Pak1 mediates dissociation of Rac GTPase.
pubmed:affiliation
Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't