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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0040715,
umls-concept:C0086597,
umls-concept:C0162638,
umls-concept:C0205263,
umls-concept:C0599718,
umls-concept:C0599813,
umls-concept:C0599893,
umls-concept:C1120843,
umls-concept:C1334482,
umls-concept:C1522702
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pubmed:issue |
1
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pubmed:dateCreated |
2004-6-25
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pubmed:abstractText |
The signaling pathways mediating nitric oxide production and apoptosis in pancreatic beta-cells are incompletely characterized. We report here that the inhibitor of p38 MAPK (p38), SB203580 (10-100 microM) inhibits interleukin-1beta (IL-1beta)-induced nitric oxide production in rat insulin-producing RINm5F cells. SB203580 also counteracts apoptosis induced by a combination of IL-1beta and interferon-gamma. However, the contribution by p38 to the induction of inducible nitric oxide synthase (iNOS) and apoptosis is independent of NF-kappaB nuclear translocation since SB203580 does not prevent IL-1beta-induced DNA-binding of this transcription factor. Furthermore, SB203580 alone leads to phosphorylation of JNK2 which may reflect inhibition of a p38-activated phosphatase. It is concluded that p38 mediates cytokine-induced iNOS-induction and apoptosis independently of NF-kappaB translocation. Moreover, a preventive effect on iNOS induction and apoptosis by inhibition of p38 may be partly masked due to simultaneous activation of JNK2 in pancreatic RINm5F cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/SB 203580,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:issn |
1148-5493
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47-52
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15217752-Animals,
pubmed-meshheading:15217752-Antineoplastic Agents,
pubmed-meshheading:15217752-Apoptosis,
pubmed-meshheading:15217752-Cell Line, Tumor,
pubmed-meshheading:15217752-Enzyme Activation,
pubmed-meshheading:15217752-Enzyme Inhibitors,
pubmed-meshheading:15217752-Imidazoles,
pubmed-meshheading:15217752-Interferon-gamma,
pubmed-meshheading:15217752-Interleukin-1,
pubmed-meshheading:15217752-Islets of Langerhans,
pubmed-meshheading:15217752-Mitogen-Activated Protein Kinase 9,
pubmed-meshheading:15217752-NF-kappa B,
pubmed-meshheading:15217752-Nitric Oxide Synthase,
pubmed-meshheading:15217752-Nitric Oxide Synthase Type II,
pubmed-meshheading:15217752-Phosphorylation,
pubmed-meshheading:15217752-Pyridines,
pubmed-meshheading:15217752-Rats,
pubmed-meshheading:15217752-Signal Transduction,
pubmed-meshheading:15217752-p38 Mitogen-Activated Protein Kinases
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pubmed:articleTitle |
p38 MAPK inhibits JNK2 and mediates cytokine-activated iNOS induction and apoptosis independently of NF-KB translocation in insulin-producing cells.
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pubmed:affiliation |
Department of Medical Cell Biology, Uppsala University, Biomedicum, PO Box 571, S-751 23 Uppsala, Sweden. Johan.Saldeen@medcellbiol.uu.se
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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