Source:http://linkedlifedata.com/resource/pubmed/id/15214028
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2004-6-23
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pubmed:abstractText |
To investigate the influence of avidity on T cell activation in vitro and in vivo, we analyzed T cells from St40 and St42 mice, which express the same transgenic TCR specific for an E1a-derived epitope of adenovirus type 5 with different expression levels and therefore different avidities. Splenocytes from both strains showed comparable cytolytic activities and required identical peptide concentrations for efficient target cell lysis and up-regulation of activation markers. However, the kinetics of CD25 up-regulation were strikingly different: whereas the majority of the high-avidity St42 T cells up-regulated the IL-2Ralpha chain within a few hours, low-avidity St40 T cells expressed only 50% of the CD25 of high-avidity T cells after 2 days. In addition, low-avidity T cells proliferated poorly and displayed impaired secretion of IL-2 and IFN-gamma. Similar results were seen with high-avidity St42 T cells stimulated with a partial agonistic peptide. Upon adoptive transfer and subsequent immunization with adenovirus, both high- and low-avidity T cells expanded, but St40 T cells were undetectable 10 days after immunization. Our model system now allows analysis of whether T cells with identical specificities but different avidities influence each other during activation and homeostatic proliferation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-2980
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pubmed:author |
pubmed-author:Bien-GräterUrsulaU,
pubmed-author:GünderMarcM,
pubmed-author:HofmannMatthiasM,
pubmed-author:OffringaRienkR,
pubmed-author:RadsakMarkusM,
pubmed-author:RammenseeHans-GeorgHG,
pubmed-author:RechtsteinerGerdG,
pubmed-author:SchildHansjörgH,
pubmed-author:ToesRené E MRE,
pubmed-author:WiemannKatrinK
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1798-806
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pubmed:meshHeading |
pubmed-meshheading:15214028-Adenoviridae,
pubmed-meshheading:15214028-Adoptive Transfer,
pubmed-meshheading:15214028-Animals,
pubmed-meshheading:15214028-Biological Markers,
pubmed-meshheading:15214028-Cell Division,
pubmed-meshheading:15214028-Cells, Cultured,
pubmed-meshheading:15214028-Cytokines,
pubmed-meshheading:15214028-Immunization,
pubmed-meshheading:15214028-Lymphocyte Activation,
pubmed-meshheading:15214028-Mice,
pubmed-meshheading:15214028-Mice, Transgenic,
pubmed-meshheading:15214028-Receptors, Antigen, T-Cell,
pubmed-meshheading:15214028-Receptors, Interleukin-2,
pubmed-meshheading:15214028-Spleen,
pubmed-meshheading:15214028-T-Lymphocytes, Cytotoxic
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pubmed:year |
2004
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pubmed:articleTitle |
T cell avidity determines the level of CTL activation.
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pubmed:affiliation |
Institute for Cell Biology, Department of Immunology, Eberhard-Karls-University, Tübingen, Germany.
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pubmed:publicationType |
Journal Article
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