Linked Life Data

15212715

Source:http://linkedlifedata.com/resource/pubmed/id/15212715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-6-23
pubmed:abstractText
In this study, we compared dendritic cells (DCs) differentiated from positively selected monocytes (CD14-DCs) to DCs differentiated from adherence-selected monocytes (adh-DCs) with emphasis on lentiviral transduction. Using a second-generation, triple-helix containing, self-inactivating lentiviral vector at a multiplicity of infection (MOI) of 15, we observed enhanced transduction of CD14-DCs (72.8 +/- 5.3%, mean fluorescence intensity [MFI] = 166 +/- 76) compared to adh-DCs (32.3 +/- 13.1%, MFI = 119 +/- 76, n = 5). More importantly, the efficiency to transduce adh-DCs was significantly increased when monocytes were incubated with antiCD14 antibody coupled beads, anti-CD14 antibodies, or lipopolysaccharide (LPS), reaching transduction efficiencies up to 86.6%, 53.3%, and 80.9%, respectively. We showed that this enhanced transduction was correlated to an activation of the monocytes, characterized by the up regulation of the cytokines interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha and the de novo synthesis of IL-6 and IL-10. However, the enhanced transduction of immature CD14-DCs was not correlated with a progression in the cell cycle from G(0) to G(1). We further showed that CD14-DCs were phenotypically comparable to adh-DCs. Functional analysis revealed that there were no differences in allostimulatory capacity, production of IL-12 p70 on CD40 ligation or expression of IL-1beta, IL-6, IL-8, IL-10, IL-12, and TNF-alpha as evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR). Finally, we showed that lentivirally transduced CD14-DCs were equally capable as adh-DCs in stimulating MAGE-A3 antigen-specific CD4(+) and CD8(+) T cells in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1043-0342
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
562-73
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15212715-Antibodies, Monoclonal, pubmed-meshheading:15212715-Antigens, CD14, pubmed-meshheading:15212715-Antigens, Neoplasm, pubmed-meshheading:15212715-CD4-Positive T-Lymphocytes, pubmed-meshheading:15212715-CD8-Positive T-Lymphocytes, pubmed-meshheading:15212715-Cell Cycle, pubmed-meshheading:15212715-Cell Differentiation, pubmed-meshheading:15212715-Cytokines, pubmed-meshheading:15212715-Dendritic Cells, pubmed-meshheading:15212715-Gene Transfer Techniques, pubmed-meshheading:15212715-Genetic Vectors, pubmed-meshheading:15212715-Humans, pubmed-meshheading:15212715-Immunophenotyping, pubmed-meshheading:15212715-Lentivirus, pubmed-meshheading:15212715-Leukocytes, Mononuclear, pubmed-meshheading:15212715-Lipopolysaccharides, pubmed-meshheading:15212715-Lymphocyte Activation, pubmed-meshheading:15212715-Monocytes, pubmed-meshheading:15212715-Neoplasm Proteins, pubmed-meshheading:15212715-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15212715-Transduction, Genetic
pubmed:year
2004
pubmed:articleTitle
Activation of monocytes via the CD14 receptor leads to the enhanced lentiviral transduction of immature dendritic cells.
pubmed:affiliation
Laboratory of Molecular and Cellular Therapy, Department of Physiology and Immunology, Medical School of the Vrije Universiteit Brussel (V.U.B.), Laarbeeklaan 103/E, 1090 Brussels, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't