Source:http://linkedlifedata.com/resource/pubmed/id/15208679
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
35
|
| pubmed:dateCreated |
2004-8-5
|
| pubmed:abstractText |
The histological diagnosis of low-grade astrocytomas and oligodendrogliomas (WHO grade II) is often challenging, particularly in cases that show both astrocytic and oligodendroglial differentiation. We carried out gene expression profiling on 17 oligodendrogliomas (93% with LOH 1p and/or 19q) and 15 low-grade astrocytomas (71% with a TP53 mutation), using a cDNA array containing 1176 cancer-related genes. In oligodendrogliomas, 40 genes showed on average higher expression (at least a two-fold increase) than in astrocytomas, including DES, TDGF1, TGF-beta, GABA-BR1A, Histone H4, CDKN1A, PCDH43, Rho7 and Jun-D, while 39 genes were expressed at lower levels (at least a two-fold decrease), including JNK2, ITGB4, JNK3A2, RhoC, IFI-56K, AAD14 and EGFR. Immunohistochemistry revealed nuclear staining of Jun-D in oligodendrogliomas, in contrast to the immunoreactivity of cytoplasm and cell processes in low-grade astrocytomas. Partial least-squares analysis of the 79 genes at least two-fold differentially expressed between oligodendrogliomas and low-grade astrocytomas demonstrated perfect separation of oligodendrogliomas from low-grade astrocytomas and normal cerebral white matter. Clustering analysis based on the entire gene set divided the 17 subjects with oligodendrogliomas into two subgroups with significantly different survival (log-rank test, P=0.0305; survival to 5-years, 80 vs 0%, P=0.048). These results demonstrate that oligodendrogliomas and low-grade astrocytomas differ in their gene expression profiles, and that there are subgroups of oligodendroglioma with distinct expression profiles related to clinical outcome.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0950-9232
|
| pubmed:author |
pubmed-author:Fevre-MontangeMichelleM,
pubmed-author:HeppnerFrank LFL,
pubmed-author:HuangHervéH,
pubmed-author:JouvetAnneA,
pubmed-author:KleihuesPaulP,
pubmed-author:LazaridisEmmanuel NEN,
pubmed-author:OhgakiHirokoH,
pubmed-author:OkamotoYoshikazuY,
pubmed-author:VitalAnneA,
pubmed-author:YokooHideakiH,
pubmed-author:YonekawaYasuhiroY
|
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6012-22
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15208679-Adult,
pubmed-meshheading:15208679-Astrocytoma,
pubmed-meshheading:15208679-Brain,
pubmed-meshheading:15208679-Brain Neoplasms,
pubmed-meshheading:15208679-Disease Progression,
pubmed-meshheading:15208679-Female,
pubmed-meshheading:15208679-Gene Expression Profiling,
pubmed-meshheading:15208679-Genes, p53,
pubmed-meshheading:15208679-Humans,
pubmed-meshheading:15208679-Loss of Heterozygosity,
pubmed-meshheading:15208679-Male,
pubmed-meshheading:15208679-Middle Aged,
pubmed-meshheading:15208679-Mutation,
pubmed-meshheading:15208679-Oligodendroglioma,
pubmed-meshheading:15208679-Oligonucleotide Array Sequence Analysis
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Gene expression profiling and subgroup identification of oligodendrogliomas.
|
| pubmed:affiliation |
International Agency for Research on Cancer (IARC), 150 Cours Albert-Thomas, F-69372 Lyon Cedex 08, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|