Source:http://linkedlifedata.com/resource/pubmed/id/15208644
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
2004-8-17
|
| pubmed:abstractText |
Hepatitis C virus (HCV) frequently causes chronic liver disease. The cause of viral persistence might be an inappropriate type I interferon (IFN) induction. To analyze the host's IFN response in chronic hepatitis C, we measured the transcription level of type I IFN genes as well as type I IFN-regulated genes in liver tissue and corresponding blood samples from patients with chronic hepatitis C, nonviral liver diseases, and a suspected but later excluded liver disease. Competitive and real-time RT-PCR assays were used to quantify the messenger RNA (mRNA) levels of all known IFN-alpha, IFN-beta, and IFN-lambda genes and those of some IFN-regulated genes. We failed to detect any hepatic type I IFN mRNA induction, although liver tissue of chronic hepatitis C patients contained high numbers of some type I IFN-inducible effector mRNA molecules. Analysis of peripheral blood samples, however, showed a clear type I IFN induction. Parallel experiments employing HCV replicon cell lines revealed that replication of HCV RNA is not sufficient to induce any type I IFN nor to induce directly type I IFN-regulated genes such as MxA. In conclusion, our data provide evidence for the absence of an induction of type I IFN genes by HCV in the human liver and argue for a further development of type I IFN-based therapies.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0023-6837
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
84
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1148-59
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15208644-Adolescent,
pubmed-meshheading:15208644-Adult,
pubmed-meshheading:15208644-Aged,
pubmed-meshheading:15208644-Carcinoma, Hepatocellular,
pubmed-meshheading:15208644-Cell Line, Tumor,
pubmed-meshheading:15208644-Female,
pubmed-meshheading:15208644-Gene Expression Regulation, Viral,
pubmed-meshheading:15208644-Hepacivirus,
pubmed-meshheading:15208644-Hepatitis C, Chronic,
pubmed-meshheading:15208644-Hepatocytes,
pubmed-meshheading:15208644-Humans,
pubmed-meshheading:15208644-Interferon Type I,
pubmed-meshheading:15208644-Liver,
pubmed-meshheading:15208644-Male,
pubmed-meshheading:15208644-Middle Aged,
pubmed-meshheading:15208644-RNA, Messenger,
pubmed-meshheading:15208644-RNA, Viral,
pubmed-meshheading:15208644-Replicon,
pubmed-meshheading:15208644-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15208644-Transcription, Genetic,
pubmed-meshheading:15208644-Virus Replication
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Interferon type I gene expression in chronic hepatitis C.
|
| pubmed:affiliation |
Division of Gastroenterology and Endocrinology, Department of Internal Medicine, Georg-August-Universität, Göttingen, Germany. smihm@med.uni-goettingen.de
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|