Source:http://linkedlifedata.com/resource/pubmed/id/15203285
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2004-6-18
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| pubmed:abstractText |
The novel multiple myeloma (MM) cell line MOLP-8 carrying the t(11;14) (q13;q32) was established from the peripheral blood of a 52-year-old Japanese male patient with Bence-Jones delta/lambda type MM (stage IIIA with hyperammonemia). The growth of MOLP-8 cells is constitutively independent of exogenous growth factors or feeder cells. MOLP-8 cells grow mainly as free floating single cells and slightly adherent on the bottom of the plastic culture flask. Wright-Giemsa-stained MOLP-8 cells show the typical plasma cell morphology with abundant cytoplasm, heterogeneous cell size and one to three nuclei. The immunoprofile of MOLP-8 corresponds to that seen typically in primary MM cells: positive for cytoplasmic immunoglobulin (Ig) delta/lambda chains, CD10, CD29, CD38, CD40, CD44, CD49b, CD49d, CD54, CD56, CD58, CD71, CD138 and PCA-1; the cells were negative for surface Igs and various other B-cell, T-cell and myelomonocyte-associated immunomarkers. CD28 became positive after co-culture of MOLP-8 cells with bone marrow adherent stromal (BST) feeder cells for a week. About 30% of MOLP-8 cells adhered strongly to the BST cells, but the cellular adhesion was clearly inhibited by addition of either anti-CD29 or anti-CD106 monoclonal antibody, suggesting a specific cellular adhesion through alpha4beta1-integrin-VCAM-1 interaction. The novel MOLP-8 cell line together with the present myeloma cell lines will present useful model systems in the investigation of the biology of MM.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin delta-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin lambda-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Integrins,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0145-2126
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
28
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
869-77
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| pubmed:dateRevised |
2005-11-17
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| pubmed:meshHeading |
pubmed-meshheading:15203285-Antigens, CD,
pubmed-meshheading:15203285-Antigens, CD28,
pubmed-meshheading:15203285-Antigens, Surface,
pubmed-meshheading:15203285-Cell Adhesion,
pubmed-meshheading:15203285-Cell Division,
pubmed-meshheading:15203285-Chromosomes, Human, Pair 11,
pubmed-meshheading:15203285-Chromosomes, Human, Pair 14,
pubmed-meshheading:15203285-Humans,
pubmed-meshheading:15203285-Immunoglobulin delta-Chains,
pubmed-meshheading:15203285-Immunoglobulin lambda-Chains,
pubmed-meshheading:15203285-Integrins,
pubmed-meshheading:15203285-Karyotyping,
pubmed-meshheading:15203285-Male,
pubmed-meshheading:15203285-Middle Aged,
pubmed-meshheading:15203285-Multiple Myeloma,
pubmed-meshheading:15203285-Plasma Cells,
pubmed-meshheading:15203285-Stromal Cells,
pubmed-meshheading:15203285-Translocation, Genetic,
pubmed-meshheading:15203285-Tumor Cells, Cultured,
pubmed-meshheading:15203285-Vascular Cell Adhesion Molecule-1
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| pubmed:year |
2004
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| pubmed:articleTitle |
Induction of CD28 on the new myeloma cell line MOLP-8 with t(11;14)(q13;q32) expressing delta/lambda type immunoglobulin.
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| pubmed:affiliation |
Fujisaki Cell Center, Hayashibara Biochemical Labs., Inc., 675-1 Fujisaki, Okayama 702-8006, Japan. yomatsuo@hayashibara.co.jp
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| pubmed:publicationType |
Journal Article
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