Linked Life Data

15203218

Source:http://linkedlifedata.com/resource/pubmed/id/15203218

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-6-18
pubmed:abstractText
The rapid amplification of cDNA ends (RACE) procedure is a widely used PCR-based method to clone the cDNA ends of mRNA transcripts. Current RACE methods often produce a high background of nonspecific PCR products, which can exclude the identification of the target cDNA of interest. We describe here an improved RACE procedure using circular cDNA templates and demonstrate the successful extension cloning of 4406 cDNAs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0888-7543
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Elsevier Inc.
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-10
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes.
pubmed:affiliation
Incyte Corporation, Palo Alto, CA 94304, USA. gfu@incyte.com
pubmed:publicationType
Journal Article