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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-6-16
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pubmed:abstractText |
Arabidopsis PsbQ, encoding a 16 kDa protein of the oxygen-evolving complex, is regulated by light and is expressed preferentially in leaf tissues. To analyze the components required for light-regulated and organ-specific expression of PsbQA, several promoter constructs were generated and expressed in tobacco. The 2.2 kb promoter could confer organ-specific expression of the reporter gene, whereas regulatory elements for light-dependent induction could not be located within this promoter and the transcribed region extending up to a second exon, represented by a genomic fragment encompassing the gene. The genomic fragment representing the transcribed region, however, could confer light regulation even on a constitutive promoter, as observed by steady-state mRNA analysis in T0 and T1 tobacco plants. The results obtained have led to the conclusion that regulatory elements for organ-specificity mainly reside in the promoter region whereas the transcribed region of the gene has an important role in light regulation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0962-8819
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
97-108
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15198198-5' Untranslated Regions,
pubmed-meshheading:15198198-Arabidopsis Proteins,
pubmed-meshheading:15198198-Blotting, Northern,
pubmed-meshheading:15198198-Caulimovirus,
pubmed-meshheading:15198198-Gene Expression Regulation, Developmental,
pubmed-meshheading:15198198-Gene Expression Regulation, Plant,
pubmed-meshheading:15198198-Genes, Reporter,
pubmed-meshheading:15198198-Glucuronidase,
pubmed-meshheading:15198198-Light,
pubmed-meshheading:15198198-Organ Specificity,
pubmed-meshheading:15198198-Photosynthesis,
pubmed-meshheading:15198198-Photosystem II Protein Complex,
pubmed-meshheading:15198198-Plants, Genetically Modified,
pubmed-meshheading:15198198-Promoter Regions, Genetic,
pubmed-meshheading:15198198-RNA, Messenger,
pubmed-meshheading:15198198-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:15198198-Tobacco,
pubmed-meshheading:15198198-Transcription, Genetic
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pubmed:year |
2004
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pubmed:articleTitle |
Analysis of Arabidopsis PsbQA gene expression in transgenic tobacco reveals differential role of its promoter and transcribed region in organ-specific and light-mediated regulation.
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pubmed:affiliation |
Centre for Plant Molecular Biology, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi-110021, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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