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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-6-15
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pubmed:abstractText |
Organ development is a complex process involving the coordination of cell proliferation, differentiation, and morphogenetic events. Using a screen to identify genes that function coordinately with lin-35/Rb during animal development, we have isolated a weak loss-of-function (LOF) mutation in pha-1. lin-35; pha-1 double mutants are defective at an early step in pharyngeal morphogenesis leading to an abnormal pharyngeal architecture. pha-1 is also synthetically lethal with other class B synthetic multivulval (SynMuv) genes including the C. elegans E2F homolog, efl-1. Reporter analyses indicate that pha-1 is broadly expressed during embryonic development and that its functions reside in the cytoplasm. We also provide genetic and phenotypic evidence to support the model that PHA-1, a novel protein, and UBC-18, a ubiquitin-conjugating enzyme that we have previously shown to function with lin-35 during pharyngeal development, act in parallel pathways to regulate the activity of a common cellular target.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0012-1606
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11-25
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15196946-Animals,
pubmed-meshheading:15196946-Branchial Region,
pubmed-meshheading:15196946-Caenorhabditis elegans,
pubmed-meshheading:15196946-Caenorhabditis elegans Proteins,
pubmed-meshheading:15196946-Crosses, Genetic,
pubmed-meshheading:15196946-Gene Expression Regulation, Developmental,
pubmed-meshheading:15196946-Genes, Reporter,
pubmed-meshheading:15196946-Green Fluorescent Proteins,
pubmed-meshheading:15196946-Ligases,
pubmed-meshheading:15196946-Luminescent Proteins,
pubmed-meshheading:15196946-Models, Biological,
pubmed-meshheading:15196946-Morphogenesis,
pubmed-meshheading:15196946-Mutation,
pubmed-meshheading:15196946-Phenotype,
pubmed-meshheading:15196946-Polymorphism, Single Nucleotide,
pubmed-meshheading:15196946-RNA Interference,
pubmed-meshheading:15196946-Repressor Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
The coordinate regulation of pharyngeal development in C. elegans by lin-35/Rb, pha-1, and ubc-18.
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pubmed:affiliation |
Department of Molecular Biology, College of Agriculture, University of Wyoming, Laramie 82071-3944, USA. davidfay@uwyo.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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