Linked Life Data

15196496

Source:http://linkedlifedata.com/resource/pubmed/id/15196496

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-6-15
pubmed:abstractText
The understanding of bacterial pathogenesis is dependent on techniques that elucidate the underlying genetic and biochemical mechanisms. To study the mechanism of bacterial survival and proliferation within host cells we need accurate tools that tell us what is occurring within the infecting organism. It has now become possible to determine the transcriptional status of in vivo-derived bacteria at the level of the whole genome. Such expression profiles serve as a monitor of the host cell environment as well as an indicator of the bacterial adaptation to its intracellular niche. Here, we review the methods used to produce microarray data for defining the bacterial intracellular transcriptome, and examine the pitfalls in extracting bacterial RNA from the infected host compartment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1369-5274
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Benefits and pitfalls of using microarrays to monitor bacterial gene expression during infection.
pubmed:affiliation
Molecular Microbiology Group, Institute of Food Research, Norwich Research Park, Norwich, NR4 7UA, United Kingdom. jay.hinton@bbsrc.ac.uk
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't