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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2004-6-15
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pubmed:abstractText |
CD1 molecules are non-polymorphic major histocompatibility complex class I-related proteins that bind and present glycolipid antigens to T-cell antigen receptors (TCR) expressed by alphabeta T cells or natural killer-like T cells (NKT). Anti-metastatic properties of NKT cells reactive to the CD1d-binding antigen alpha-galactosylceramide (alpha-GalCer) are now being explored as a contributor to tumour cell killing. In this study, we tested the hypothesis that presentation of alpha-GalCer by murine CD1d (mCD1d) to mCD1d-restricted NKT cells was facilitated by plasma membrane glycolipid rafts. Confocal microscopy of mCD1d-transfected A20 B cells (A20mCD1d) demonstrated that mCD1d was raft-localized. This observation was confirmed by immunoblotting of raft fractions isolated on sucrose density gradients. Raft disruption by the cholesterol-binding agent nystatin, or short-chain ceramides, inhibited presentation of low concentrations of alpha-GalCer to NKT cells. Inhibition of antigen presentation was reversed by treatment of A20mCD1d cells with higher alpha-GalCer concentrations, or removal of raft-disrupting agents. These data indicate that partitioning of mCD1d into membrane rafts increases the capacity of antigen-presenting cells to present limiting quantities of glycolipid antigens, perhaps by stabilizing mCD1d/antigen structures on the plasma membrane and optimizing TCR engagement on NKT cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10201072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10358761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10359095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10428838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10459018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10523605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10587346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10593509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10779793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10786796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-10963678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11158680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11208113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11248809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11544296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11722638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11731798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11754812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11830474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11869887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11927549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11938350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-11956292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-12023895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-12176894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-12376543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-12515827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-12538693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-12637320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-12724530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-12829737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-2415625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-3141504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-7982998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9020144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9047240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9177342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9261060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9531266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9655486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9738502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9741631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9759842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9826697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9874567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15196206-9880557
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
112
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
386-96
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15196206-Animals,
pubmed-meshheading:15196206-Antigen Presentation,
pubmed-meshheading:15196206-Antigens, CD1,
pubmed-meshheading:15196206-Antigens, CD1d,
pubmed-meshheading:15196206-B-Lymphocytes,
pubmed-meshheading:15196206-Cell Line,
pubmed-meshheading:15196206-Cell Membrane,
pubmed-meshheading:15196206-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15196206-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15196206-Galactosylceramides,
pubmed-meshheading:15196206-Glycolipids,
pubmed-meshheading:15196206-Immunoblotting,
pubmed-meshheading:15196206-Interleukin-2,
pubmed-meshheading:15196206-Killer Cells, Natural,
pubmed-meshheading:15196206-Mice,
pubmed-meshheading:15196206-Microscopy, Confocal
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pubmed:year |
2004
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pubmed:articleTitle |
Presentation of alpha-galactosylceramide by murine CD1d to natural killer T cells is facilitated by plasma membrane glycolipid rafts.
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pubmed:affiliation |
Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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