Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2004-6-15
pubmed:abstractText
RNA interference has become a powerful tool for silencing of gene expression in mammals and plants. To determine the effect of Smad3 on transforming growth factor-beta signaling, we constructed a small interfering RNA (siRNA) targeted to Smad3. This siRNA inhibited expression of the endogenous Smad3 leading to the prevention of nuclear localization of Smad3. Further, Smad3 siRNA prevented not only anti-proliferative activity of TGF-beta1 but also TGF-beta1-inducible promoter activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0141-5492
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
699-703
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15195967-Animals, pubmed-meshheading:15195967-Cell Line, Tumor, pubmed-meshheading:15195967-Cell Nucleus, pubmed-meshheading:15195967-Cell Proliferation, pubmed-meshheading:15195967-Cytoplasm, pubmed-meshheading:15195967-DNA-Binding Proteins, pubmed-meshheading:15195967-Luciferases, pubmed-meshheading:15195967-Mice, pubmed-meshheading:15195967-Microscopy, Fluorescence, pubmed-meshheading:15195967-Plasmids, pubmed-meshheading:15195967-Promoter Regions, Genetic, pubmed-meshheading:15195967-RNA, Small Interfering, pubmed-meshheading:15195967-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15195967-Signal Transduction, pubmed-meshheading:15195967-Smad3 Protein, pubmed-meshheading:15195967-Thymidine, pubmed-meshheading:15195967-Trans-Activators, pubmed-meshheading:15195967-Transfection, pubmed-meshheading:15195967-Transforming Growth Factor beta
pubmed:year
2004
pubmed:articleTitle
Small interfering RNA (siRNA) targetted to Smad3 inhibits transforming growth factor-beta signaling.
pubmed:affiliation
Department of Microbiology, College of Natural Sciences, Kangwon National University, Chunchon 200-701, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't