Linked Life Data

15193776

Source:http://linkedlifedata.com/resource/pubmed/id/15193776

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-6-14
pubmed:abstractText
Much attention has been paid to the membrane microdomain enriched in cholesterol and sphingolipids called raft. In the central nervous system, however, the physiological role of this domain is not so evident at present, partly because of the complexity of the protein components in the raft fraction. In this study we surveyed ATPase activities in the raft fraction obtained from the synaptic plasma membrane of rat brain and found the enrichment of an ethacrynic acid-sensitive ATPase (Cl(-)-pump) activity. Immunoprecipitation experiments using antibodies to raft-localized proteins showed the co-precipitation of the ATPase activity with NAP-22, a major raft-localized protein. This result suggests the participation of the raft in the regulation of ion transport in addition to the presence of heterogeneity of raft domains in neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0304-3940
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Elsevier Ireland Ltd.
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
362
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
158-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Localization of the Cl(-)-ATPase activity on NAP-22 enriched membrane microdomain (raft) of rat brain.
pubmed:affiliation
Division of Bioinformation, Department of Biosystems Science, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan. smaekawa@kobe-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't