Linked Life Data

15193742

Source:http://linkedlifedata.com/resource/pubmed/id/15193742

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-6-14
pubmed:abstractText
A simple, reliable and reproducible method, based on capillary zone electrophoresis with amperometric detection (CZE-AD), has been developed for simultaneous determination of five active ingredients in complicated traditional Chinese medicines including chlorogenic acid, caffeic acid, aloe-emodin, emodin and rhein. A carbon-disk electrode was used as working electrode. The optimal conditions of CZE detection were 30 mM borate solution (pH 9.5) as running buffer, 18 kV as separation voltage and 1.00 V (versus Ag/AgCl) as detection potential. There was excellent linearity between current response and analyte concentration over two orders of magnitude, while the limits of detection were 1.1 x 10(-7), 3.0 x 10(-7), 1.5 x 10(-7), 2.9 x 10(-7), 1.4 x 10(-6) mol/l for aloe-emodin, emodin, rhein, chlorogenic acid and caffeic acid, respectively (S/N = 3). The utility of this method was demonstrated by monitoring a kind of complicated Chinese medicine named Huangdan Yinchen Keli. Two samples manufactured by different companies were monitored with satisfactory results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0731-7085
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Elsevier B.V.
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
959-64
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Determination of active ingredients in Huangdan Yinchen Keli by CZE with amperometric detection.
pubmed:affiliation
Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
pubmed:publicationType
Journal Article