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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1992-10-7
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pubmed:abstractText |
Depolarization-induced increases in cytoplasmic and intranuclear Ca2+ were visualized in adult mammalian dorsal root ganglion (DRG) neurons during different stages of neurite extension by using confocal laser scanning microscopy and the long-wavelength Ca2+ indicator dye fluo 3-AM (acetoxymethyl ester of fluo 3). In neurons beginning to extend neurites, depolarization led to pronounced increases in nuclear and nucleolar Ca2+ levels severalfold greater than corresponding increases in the cytoplasm. The nucleolar Ca2+ signal often exceeded that of the nucleus, indicating regional heterogeneity of the nucleus. The subcellular calcium transients were dependent on extracellular Ca2+ and the level of depolarization, indicating the importance of transmembrane Ca2+ fluxes in triggering the nuclear events. After neurite extension, the nuclear Ca2+ signals were attenuated and never exceeded cytoplasmic levels. These results indicate that activity-dependent modulation of intranuclear Ca2+ levels is greater in DRG neurons during early neurite extension. Given the importance of Ca2+ in gene expression, the results may be relevant to Ca(2+)-dependent nuclear events responsible for axonal regeneration.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-1741596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-1742025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2004666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2010811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2154851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2157471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2167857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2354502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2426600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2479148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-2498309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-3103219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-3749894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-49355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518824-5289222
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7978-82
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1518824-Animals,
pubmed-meshheading:1518824-Calcium,
pubmed-meshheading:1518824-Cell Differentiation,
pubmed-meshheading:1518824-Cell Nucleus,
pubmed-meshheading:1518824-Cells, Cultured,
pubmed-meshheading:1518824-Female,
pubmed-meshheading:1518824-Ganglia, Spinal,
pubmed-meshheading:1518824-Membrane Potentials,
pubmed-meshheading:1518824-Neurites,
pubmed-meshheading:1518824-Rats,
pubmed-meshheading:1518824-Rats, Inbred Strains
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pubmed:year |
1992
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pubmed:articleTitle |
Intranuclear Ca2+ transients during neurite regeneration of an adult mammalian neuron.
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pubmed:affiliation |
Department of Neurology, Yale University School of Medicine, New Haven, CT 06510.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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