Linked Life Data

1518809

Source:http://linkedlifedata.com/resource/pubmed/id/1518809

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
1992-10-7
pubmed:abstractText
We used mixed, mutagenic oligonucleotides to create single amino acid substitutions in the bacterial chemoreceptor Trg. Mutagenesis was directed at a 20-residue segment of the periplasmic domain implicated in ligand recognition. Transmembrane signaling by the mutant receptors was assayed in vivo by monitoring adaptational covalent modification. Among 20 functionally altered but stable receptors there were two distinct signaling phenotypes. Insensitive receptors did not signal upon stimulation and thus appeared defective in productive ligand interaction. Mimicked-occupancy receptors exhibited transmembrane signaling without ligand. Many mimicked-occupancy receptors produced additional signaling upon ligand binding and in appropriate conditions mediated effective chemotaxis; most insensitive receptors did not. Like normal receptors with one binding site occupied, mimicked-occupancy proteins adapted to persistent transmembrane signaling by increased methylation and thus could respond to other stimuli. Signaling phenotypes were strikingly segregated by residue position. Substitutions mimicking ligand occupancy occurred in half the segment, and those creating insensitive phenotypes occurred in the other half. These observations could be related to the three-dimensional structure of the periplasmic domain of the Tar(s) chemoreceptor. Insensitive substitutions occurred near the distal end of helix 1, where bulky protein ligands could interact; occupancy-mimicking substitutions were on the same helix at positions buried in the subunit interface between helices 1 and 1'. Thus perturbation of the interface induced transmembrane signaling, implicating changes at that interface in signal transduction, a conclusion consistent with differences in crystal structures of unoccupied and ligand-occupied Tar(s).
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-1537797, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-1660187, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-1661030, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-1883200, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-2104606, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-2646634, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3017920, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3032955, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3041407, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3049535, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3049536, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3052756, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3056621, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3076088, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3087946, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3096976, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3139636, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3282853, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3297352, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3323813, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3803923, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-3881765, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-4359647, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-6260957, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-6310323, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-6446711, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-6782079, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-6993007, http://linkedlifedata.com/resource/pubmed/commentcorrection/1518809-6994098
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
89
pubmed:geneSymbol
trg, tsr
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7890-4
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Ligand occupancy mimicked by single residue substitutions in a receptor: transmembrane signaling induced by mutation.
pubmed:affiliation
Departments of Genetics, Washington State University, Pullman 99164-4660.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.