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rdf:type |
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lifeskim:mentions |
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0085295,
umls-concept:C0086418,
umls-concept:C1155046,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1533691,
umls-concept:C1540293,
umls-concept:C2349975
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pubmed:issue |
6
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pubmed:dateCreated |
2004-6-9
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pubmed:abstractText |
To explore the biofunctions of human B7-H3 on activated T lymphocyte, the gene of human B7-H3 encoding the extracellular region (IgV-like and IgC-like domains) was obtained by RT-PCR from human lung cells and subcloned into the prokaryotic expression vector pGEX-5X-3 to express glutathione S-transferase (GST) fusion protein. A 49 kD fusion protein (named as GST/hB7-H3 hereafter) was induced by IPTG and purified by standard methods reported in prokaryotic system. In the presence of the first signal imitated by anti-CD3 monoclonal antibody, T lymphocyte proliferation was observed by incubating purified T cells with soluble GST/hB7-H3 fusion protein by MTT assay. The concentrations of IFN-gamma and IL-10 in the supernatants of T cells were determined by ELISA. The results showed that the GST/hB7-H3 protein produced in bacteria had modest biological activities to proliferate the T lymphocyte and enhance IFN-gamma as well as IL-10 secretion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/B7 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/CD276 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Isopropyl Thiogalactoside,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1672-9145
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
430-6
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15188059-Antibodies, Monoclonal,
pubmed-meshheading:15188059-Antigens, CD,
pubmed-meshheading:15188059-Antigens, CD80,
pubmed-meshheading:15188059-B7 Antigens,
pubmed-meshheading:15188059-Blotting, Western,
pubmed-meshheading:15188059-Cell Line,
pubmed-meshheading:15188059-Cell Proliferation,
pubmed-meshheading:15188059-Cloning, Molecular,
pubmed-meshheading:15188059-Coloring Agents,
pubmed-meshheading:15188059-Cytokines,
pubmed-meshheading:15188059-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15188059-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15188059-Escherichia coli,
pubmed-meshheading:15188059-Genetic Vectors,
pubmed-meshheading:15188059-Glutathione Transferase,
pubmed-meshheading:15188059-Humans,
pubmed-meshheading:15188059-Interferon-gamma,
pubmed-meshheading:15188059-Interleukin-10,
pubmed-meshheading:15188059-Isopropyl Thiogalactoside,
pubmed-meshheading:15188059-Protein Structure, Tertiary,
pubmed-meshheading:15188059-Receptors, Immunologic,
pubmed-meshheading:15188059-Recombinant Fusion Proteins,
pubmed-meshheading:15188059-Recombinant Proteins,
pubmed-meshheading:15188059-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15188059-T-Lymphocytes,
pubmed-meshheading:15188059-Tetrazolium Salts,
pubmed-meshheading:15188059-Thiazoles
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pubmed:year |
2004
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pubmed:articleTitle |
Human recombinant B7-H3 expressed in E. coli enhances T lymphocyte proliferation and IL-10 secretion in vitro.
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pubmed:affiliation |
Institute of Biotechnology, Soochow University, Key Laboratory of Clinical Immunology of Jiangsu Province, Suzhou 215007, China.
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pubmed:publicationType |
Journal Article,
In Vitro
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