Source:http://linkedlifedata.com/resource/pubmed/id/15185009
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
2004-8-9
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| pubmed:abstractText |
Interleukin 6 (IL-6) facilitates the differentiation of B cells to immunoglobulin-secreting cells and is reported to be a proliferative factor in some tumors. In this study, we examined IL-6 production in non-small cell lung carcinoma (NSCLC) and the proliferation of tumor cells following IL-6 treatment in vitro and in vivo. We analyzed the expression of IL-6 mRNA and protein in a series of 15 human lung cancer cell lines (four adenocarcinomas, five squamous cell carcinomas, two large cell carcinomas, and four small cell carcinomas) by reverse transcriptase polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). We established an IL-6-producing cell line (ABC-1#IL-6) by transfecting a human IL-6 cDNA into a human non-IL-6-producing NSCLC cell line (ABC-1). These two cell lines were used to determine tumor cell proliferation both in vivo and in vitro in order to clarify the effect of IL-6 on tumor growth and metastasis. Athymic nude mice, SCID mice, and BALB/c mice were subcutaneously inoculated with these two cell lines, and body weight, tumor growth, and tumor doubling time were measured. The presence of IL-6 and tumor-infiltrating lymphocytes (TILs) within tumor tissues was examined by immunohistochemical staining. RESULTS: Eight of 15 (53%) lung cancer cell lines expressed both IL-6 mRNA and protein. Tumor lesions of both cell lines developed in nude and SCID mice, although no such lesions of either cell lines developed in BALB/c mice. The tumor doubling time in nude and SCID mice was 2.97+/-1.22 days and 2.45+/-1.32 days, respectively, in mice inoculated with the cell line ABC-1#IL-6. These doubling times were statistically significantly shorter than those evident in mice inoculated with the control original ABC-1 cell line (nude, p=0.0337; SCID, p=0.0119; unpaired t-test). The rates of cell proliferation in vitro of the ABC-1#IL-6 and original ABC-1 cells lines were comparable ( p=0.1441, unpaired t-test). Immunohistochemical staining revealed strong IL-6 expression in tumors derived from the IL-6-producing cell line but not in tumors derived from the original ABC-1 cell line (both in nude and SCID mice). CONCLUSION: 53% of lung cancer cell lines produce IL-6 mRNA and protein. Although IL-6 itself does not influence tumor cell proliferation in vitro, an association between IL-6 expression and tumor proliferation was found in vivo in nude and SCID mice. An anti-IL-6 reagent could provide a novel therapeutic strategy in patients with IL-6-producing lung tumors.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0340-7004
|
| pubmed:author |
pubmed-author:ChangHaoH,
pubmed-author:FujisawaTakehikoT,
pubmed-author:HiroshimaKenzoK,
pubmed-author:IizasaToshihikoT,
pubmed-author:KohEitetsuE,
pubmed-author:MotohashiShinichiroS,
pubmed-author:NakayamaToshinoriT,
pubmed-author:OtsujiMizutoM,
pubmed-author:SuzukiMakotoM,
pubmed-author:TagawaMasatoshiM,
pubmed-author:YamajiHarukoH,
pubmed-author:YokoiSanaS
|
| pubmed:issnType |
Print
|
| pubmed:volume |
53
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
786-92
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15185009-Adenocarcinoma,
pubmed-meshheading:15185009-Animals,
pubmed-meshheading:15185009-Carcinoma, Large Cell,
pubmed-meshheading:15185009-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:15185009-Carcinoma, Small Cell,
pubmed-meshheading:15185009-Carcinoma, Squamous Cell,
pubmed-meshheading:15185009-Cell Division,
pubmed-meshheading:15185009-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15185009-Gene Expression Regulation,
pubmed-meshheading:15185009-Immunoenzyme Techniques,
pubmed-meshheading:15185009-Interleukin-6,
pubmed-meshheading:15185009-Lung Neoplasms,
pubmed-meshheading:15185009-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:15185009-Male,
pubmed-meshheading:15185009-Mice,
pubmed-meshheading:15185009-Mice, Inbred BALB C,
pubmed-meshheading:15185009-Mice, Nude,
pubmed-meshheading:15185009-Mice, SCID,
pubmed-meshheading:15185009-RNA, Messenger,
pubmed-meshheading:15185009-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15185009-Transfection,
pubmed-meshheading:15185009-Tumor Cells, Cultured
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Correlation between interleukin 6 production and tumor proliferation in non-small cell lung cancer.
|
| pubmed:affiliation |
Department of Thoracic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro
|