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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2004-6-8
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pubmed:abstractText |
The stability of cryptococcal antigen from Cryptococcus neoformans serotype A and D strains at different temperatures in serum and other solvents was studied. Samples stored at -20 or 4 degrees C had equivalent stabilities as measured by the Premier Cryptococcal Antigen kit and the Cryptococcal Antigen Latex Agglutination System (CALAS) kit. However, using the Premier Cryptococcal Antigen kit, there was a 91% loss of reactivity in samples incubated in human serum for 4 weeks at 37 degrees C. A loss of reactivity of more than 99% was observed after incubation at 45 degrees C for 4 weeks. The capsular antigen was not detected by the Premier Cryptococcal Antigen kit after 16 weeks at elevated temperatures. Antigen titers were also reduced in a latex agglutination assay (CALAS) after 4 weeks at 37 and 45 degrees C. The loss of antigen reactivity was a function of pH and temperature.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-1400951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-14715758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-1583327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-1761681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-2007642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-3235676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-6643665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-7729900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-8408579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-8789042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184471-9521136
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2786-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15184471-Antibodies, Monoclonal,
pubmed-meshheading:15184471-Antigens, Fungal,
pubmed-meshheading:15184471-Cryptococcus neoformans,
pubmed-meshheading:15184471-Humans,
pubmed-meshheading:15184471-Hydrogen-Ion Concentration,
pubmed-meshheading:15184471-Latex Fixation Tests,
pubmed-meshheading:15184471-Polysaccharides,
pubmed-meshheading:15184471-Reagent Kits, Diagnostic,
pubmed-meshheading:15184471-Temperature
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pubmed:year |
2004
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pubmed:articleTitle |
Immunoreactivity of cryptococcal antigen is not stable under prolonged incubations in human serum.
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pubmed:affiliation |
Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Ave., Golding 701, Bronx, NY 10461, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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