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rdf:type |
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lifeskim:mentions |
umls-concept:C0005576,
umls-concept:C0007413,
umls-concept:C0085470,
umls-concept:C0205173,
umls-concept:C0205390,
umls-concept:C0205463,
umls-concept:C0220781,
umls-concept:C0682929,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
6
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pubmed:dateCreated |
2004-6-8
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pubmed:abstractText |
Pseudomonas putida strain DOT-T1E is highly tolerant to organic solvents, with a logP(ow) (the logarithm of the partition coefficient of a solvent in a two-phase water-octanol system of > or =2.5. Solvent tolerant microorganisms can be exploited to develop double-phase (organic solvent and water) biotransformation systems in which toxic substrates or products are kept in the organic phase. We tested P. putida DOT-T1E tolerance to different aliphatic alcohols with a logP(ow) value between 2 and 4, such as decanol, nonanol, and octanol, which are potentially useful in biotransformations in double-phase systems in which compounds with a logP(ow) around 1.5 are produced. P. putida DOT-T1E responds to aliphatic alcohols as the second phase through cis-to-trans isomerization of unsaturated cis fatty acids and through efflux of these aliphatic alcohols via a series of pumps that also extrude aromatic hydrocarbons. These defense mechanisms allow P. putida DOT-T1E to survive well in the presence of high concentrations of the aliphatic alcohols, and growth with nonanol or decanol occurred at a high rate, whereas in the presence of an octanol double-phase growth was compromised. Our results support that the logP(ow) of aliphatic alcohols correlates with their toxic effects, as octanol (logP(ow) = 2.9) has more negative effects in P. putida cells than 1-nonanol (logP(ow) = 3.4) or 1-decanol (logP(ow) = 4). A P. putida DOT-T1E derivative bearing plasmid pWW0-xylE::Km transforms m-xylene (logP(ow) = 3.2) into 3-methylcatechol (logP(ow) = 1.8). The amount of 3-methylcatechol produced in an aliphatic alcohol/water bioreactor was 10- to 20-fold higher than in an aqueous medium, demonstrating the usefulness of double-phase systems for this particular biotransformation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-10333523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-10482510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-10648517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-10744688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-10809693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-10968630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-11251828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-11377761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-11395460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-11526042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-12142492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-12637735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-12743835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-12896994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-12957894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-13671378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-1367167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-1444381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-14506010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-2687253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-7603409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-7608060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-7764006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-7921251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-9343354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-9642183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-9658016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15184168-9783170
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3637-43
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2004
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pubmed:articleTitle |
Biotransformation in double-phase systems: physiological responses of Pseudomonas putida DOT-T1E to a double phase made of aliphatic alcohols and biosynthesis of substituted catechols.
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pubmed:affiliation |
Department of Biochemistry and Molecular and Cellular Biology of Plants, CSIC-Estación Experimental del Zaidín, E-18008 Granada, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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