Linked Life Data

15184043

Source:http://linkedlifedata.com/resource/pubmed/id/15184043

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-6-8
pubmed:abstractText
Eukaryotic translation initiation factor 2B (eIF2B) is the guanine-nucleotide exchange factor for eukaryotic initiation factor 2 (eIF2). eIF2B is a heteropentameric protein composed of alpha- subunits. The alpha, beta, and delta subunits form a regulatory subcomplex, while the gamma and form a catalytic subcomplex. Archaea possess homologues of alpha, beta, and delta subunits of eIF2B. Here, we report the three-dimensional structure of an archaeal regulatory subunit (aIF2Balpha) from the hyperthermophilic archaeon Pyrococcus horikoshii OT3 determined by X-ray crystallography at 2.2A resolution. aIF2Balpha consists of two subdomains, an N-domain (residues 1-95) and a C-domain (residues 96-276), connected by a long alpha-helix (alpha5: 78-106). The N-domain contains a five helix bundle structure, while the C-domain folds into the alpha/beta structure, thus showing similarity to D-ribose-5-phosphate isomerase structure. The presence of two molecules in the crystallographic asymmetric unit and the gel filtration analysis suggest a dimeric structure of aIF2Balpha in solution, interacting with each other by C-domains. Furthermore, the crystallographic 3-fold symmetry generates a homohexameric structure of aIF2Balpha; the interaction is primarily mediated by the long alpha-helix at the N-domains. This structure suggests an architecture of the three subunits, alpha, beta, and delta, in the regulatory subcomplex within eIF2B.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
319
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
725-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15184043-Amino Acid Sequence, pubmed-meshheading:15184043-Animals, pubmed-meshheading:15184043-Archaeal Proteins, pubmed-meshheading:15184043-Crystallography, X-Ray, pubmed-meshheading:15184043-Humans, pubmed-meshheading:15184043-Macromolecular Substances, pubmed-meshheading:15184043-Models, Molecular, pubmed-meshheading:15184043-Molecular Sequence Data, pubmed-meshheading:15184043-Peptide Initiation Factors, pubmed-meshheading:15184043-Protein Folding, pubmed-meshheading:15184043-Protein Structure, Quaternary, pubmed-meshheading:15184043-Protein Structure, Secondary, pubmed-meshheading:15184043-Protein Structure, Tertiary, pubmed-meshheading:15184043-Protein Subunits, pubmed-meshheading:15184043-Pyrococcus horikoshii, pubmed-meshheading:15184043-Sequence Alignment, pubmed-meshheading:15184043-Sequence Homology, Amino Acid
pubmed:year
2004
pubmed:articleTitle
Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B.
pubmed:affiliation
Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't