Linked Life Data

1517957

Source:http://linkedlifedata.com/resource/pubmed/id/1517957

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-10-6
pubmed:abstractText
Sporothrix schenckii conidia were induced to germinate in varying concentrations of added calcium. Calcium had a stimulatory effect on the process and a calcium concentration of 1 mM was found to be optimal. Measurements of radioactive calcium uptake confirmed that uptake was taking place during the germination of conidia to the mycelial form of the fungus. Three calcium uptake peaks were observed, the first occurred 10 min after inoculation and the other two peaks coincided with the first 2 cycles of DNA synthesis in these cells. Ionophore A23187 and cobalt sulfate inhibited germination of S. schenckii conidia and altered the normal calcium uptake pattern in these cells. On the other hand, phorbol 12-myristate-13-acetate, a stimulator of protein kinase C (PKC) activity, stimulated germination in medium without calcium. This suggests that PKC activation might account for the germination of conidia in the absence of added calcium. The effects of increasing the calcium concentration on RNA and protein synthesis were also studied. A stimulation of RNA synthesis was found at all times tested with maximum stimulation 6 h after inoculation. A stimulation in protein synthesis was also observed but only after 12 h incubation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0268-1218
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
185-95
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Effects of calcium ions on the germination of Sporothrix schenckii conidia.
pubmed:affiliation
Department of Microbiology, San Juan Bautista Medical School, University of Puerto Rico.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't