Source:http://linkedlifedata.com/resource/pubmed/id/15179526
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2004-10-13
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pubmed:abstractText |
A continuous fed-batch reactor system was developed to rapidly obtain dense chlororespiring cultures of Anaeromyxobacter dehalogenans strain 2CP-C. A syringe pump continuously delivered concentrated 2,6-dichlorophenol (50-150 mM) to an anaerobic reactor vessel at a rate that sustained linear growth but prevented the substrate toxicity of chlorophenol. Dechlorination was not significantly inhibited by end product phenol up to 8 mM. A cell density of 76.8 mg protein l(-1) was obtained in 24 h. Specific growth rates averaged 0.033 h(-1)at 50% substrate limitation, which was in agreement with the maximum specific growth rate of 0.068 h(-1). This reactor system provides an efficient, cost-effective, and convenient method to rapidly obtain dense dechlorinating biomass and is promising to accelerate investigations of enzymes involved in chlororespiration.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,6-dichlorophenol,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorine Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorophenols,
http://linkedlifedata.com/resource/pubmed/chemical/Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Phenol
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0175-7598
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
377-82
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15179526-Anaerobiosis,
pubmed-meshheading:15179526-Bacterial Proteins,
pubmed-meshheading:15179526-Biodegradation, Environmental,
pubmed-meshheading:15179526-Biomass,
pubmed-meshheading:15179526-Bioreactors,
pubmed-meshheading:15179526-Chlorine Compounds,
pubmed-meshheading:15179526-Chlorophenols,
pubmed-meshheading:15179526-Enzymes,
pubmed-meshheading:15179526-Myxococcales,
pubmed-meshheading:15179526-Phenol
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pubmed:year |
2004
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pubmed:articleTitle |
The generation of high biomass from chlororespiring bacteria using a continuous fed-batch bioreactor.
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pubmed:affiliation |
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801-2352, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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