Source:http://linkedlifedata.com/resource/pubmed/id/15175420
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001275,
umls-concept:C0001554,
umls-concept:C0013878,
umls-concept:C0025677,
umls-concept:C0026809,
umls-concept:C0086418,
umls-concept:C0205178,
umls-concept:C0520484,
umls-concept:C0600688,
umls-concept:C0699790,
umls-concept:C1145667,
umls-concept:C1167622,
umls-concept:C1280519,
umls-concept:C1517564,
umls-concept:C2349209,
umls-concept:C2825311
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pubmed:issue |
1
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pubmed:dateCreated |
2004-9-17
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pubmed:abstractText |
We previously developed a new formulation of methotrexate (MTX) that is adsorbed onto a suspension of activated carbon particles (MTX-CH) and reported the usefulness of local administration in murine tumors. The present study examines the effects of human colon carcinoma (LoVo) xenografts and the acute toxicity of MTX-CH compared with MTX aqueous solution (MTX-AQ) in mice. In therapeutic experiments, LoVo cells were implanted into the backs of BALB/c nude mice. When the cells had developed into tumors, we performed an intratumoral administration of a weekly dose of 30 mg/kg. The MTX concentration in the tumor was compared between the MTX-CH group and MTX-AQ group. In experiments on acute toxicity, MTX-CH and MTX-AQ were injected subcutaneously in BDF1 mice, and intoxication symptoms, changes in body weight, and date of death were recorded. In the therapeutic experiments, intratumoral administration of MTX-CH was much more effective in suppressing the tumor growth compared with MTX-AQ. In experiments of acute toxicity, the death time of the MTX-CH group was delayed to a greater extent, and the 50% lethal dose (LD(50)) values of MTX-CH were lower than those of MTX-AQ. The LD(50) values of MTX-CH are 75 times higher than the efficacious dose of 30 mg/kg. The present results suggest that intratumoral administration of MTX-CH is useful for local therapy and the therapeutic dose of MTX-CH can be safely injected subcutaneously.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3565
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
311
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
382-7
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pubmed:meshHeading |
pubmed-meshheading:15175420-Animals,
pubmed-meshheading:15175420-Autopsy,
pubmed-meshheading:15175420-Body Weight,
pubmed-meshheading:15175420-Charcoal,
pubmed-meshheading:15175420-Colonic Neoplasms,
pubmed-meshheading:15175420-Humans,
pubmed-meshheading:15175420-Lethal Dose 50,
pubmed-meshheading:15175420-Male,
pubmed-meshheading:15175420-Methotrexate,
pubmed-meshheading:15175420-Mice,
pubmed-meshheading:15175420-Mice, Inbred BALB C,
pubmed-meshheading:15175420-Phenotype,
pubmed-meshheading:15175420-Xenograft Model Antitumor Assays
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pubmed:year |
2004
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pubmed:articleTitle |
Intratumoral administration of methotrexate bound to activated carbon particles: antitumor effectiveness against human colon carcinoma xenografts and acute toxicity in mice.
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pubmed:affiliation |
Department of Digestive Surgery, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji Kajiicho 465, Kamigyo-ku, Kyoto 602-8566, Japan. yuen-n@koto.kpu-m.ac.jp
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pubmed:publicationType |
Journal Article
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