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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2004-6-8
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pubmed:abstractText |
Calcium is a critical mediator of many intracellular processes in eukaryotic cells. In the obligate intracellular parasite Toxoplasma gondii, for example, a rise in [Ca2+] is associated with significant morphological changes and rapid egress from host cells. To understand the mechanisms behind such dramatic effects, we isolated a mutant that is altered in its responses to the Ca2+ ionophore A23187 and found the affected gene encodes a homologue of Na+/H+ exchangers (NHEs) located on the parasite's plasma membrane. We show that in the absence of TgNHE1, Toxoplasma is resistant to ionophore-induced egress and extracellular death and amiloride-induced proton efflux inhibition. In addition, the mutant has increased levels of intracellular Ca2+, which explains its decreased sensitivity to A23187. These results provide direct genetic evidence of a role for NHE1 in Ca2+ homeostasis and important insight into how this ubiquitous pathogen senses and responds to changes in its environment.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15173192-10559194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15173192-10812075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15173192-10823923,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15173192-9480901
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:copyrightInfo |
Copyright the Rockefeller University Press
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
653-62
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15173192-Amiloride,
pubmed-meshheading:15173192-Animals,
pubmed-meshheading:15173192-Calcimycin,
pubmed-meshheading:15173192-Calcium,
pubmed-meshheading:15173192-Calcium Signaling,
pubmed-meshheading:15173192-Cell Death,
pubmed-meshheading:15173192-Cell Membrane,
pubmed-meshheading:15173192-Drug Resistance,
pubmed-meshheading:15173192-Homeostasis,
pubmed-meshheading:15173192-Ion Channels,
pubmed-meshheading:15173192-Ionophores,
pubmed-meshheading:15173192-Mutation,
pubmed-meshheading:15173192-Protons,
pubmed-meshheading:15173192-Sodium-Hydrogen Antiporter,
pubmed-meshheading:15173192-Toxoplasma
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pubmed:year |
2004
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pubmed:articleTitle |
Ionophore-resistant mutant of Toxoplasma gondii reveals involvement of a sodium/hydrogen exchanger in calcium regulation.
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pubmed:affiliation |
Department of Microbiology and Immunology, Fairchild Building D305, 300 Pasteur Dr., Stanford University School of Medicine, Stanford, CA 94305-5124, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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