Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1992-10-7
|
pubmed:abstractText |
A method for the determination of 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) by gas chromatography-mass spectrometry (GC-MS) in samples obtained from healthy subjects by nasal lavage is presented. HETEs were extracted from samples obtained by nasal lavage using C18 solid-phase cartridges. The purification of 12-HETE and 5-HETE was carried out in two consecutive steps of purification by reversed-phase high-performance liquid chromatography. Methylated and trimethylsilylated fractions were separated by GC-MS with electron-impact ionization. The production of 12-HETE by human nasal mucosa was confirmed by GC-MS.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/12-Hydroxy-5,8,10,14-eicosatetraenoi...,
http://linkedlifedata.com/resource/pubmed/chemical/5-hydroxy-6,8,11,14-eicosatetraenoic...,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyeicosatetraenoic Acids
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0021-9673
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
13
|
pubmed:volume |
575
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
143-6
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1517291-12-Hydroxy-5,8,10,14-eicosatetraenoic Acid,
pubmed-meshheading:1517291-Arachidonic Acid,
pubmed-meshheading:1517291-Chromatography, High Pressure Liquid,
pubmed-meshheading:1517291-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:1517291-Humans,
pubmed-meshheading:1517291-Hydroxyeicosatetraenoic Acids,
pubmed-meshheading:1517291-Nasal Mucosa
|
pubmed:year |
1992
|
pubmed:articleTitle |
In vivo transformation of arachidonic acid into 12-hydroxy-5,8,10,14-eicosatetraenoic acid by human nasal mucosa.
|
pubmed:affiliation |
Department of Neurochemistry, Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|