Linked Life Data

15171705

Source:http://linkedlifedata.com/resource/pubmed/id/15171705

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-6-2
pubmed:abstractText
We elucidated the structure of the principle factors regulating the initiation of the acute stress response in common carp: corticotrophin-releasing hormone (CRH), CRH-receptor 1 (CRH-R1) and CRH-binding protein (CRH-BP). Phylogenetic analyses reveal that these proteins are evolutionarily well conserved in vertebrates. CRH and CRH-BP expression are not co-localised in the same hypothalamic perikarya. On the contrary, CRH-BP expression is limited to the perimeter of the nucleus preopticus (NPO), but is abundant in other regions, including an area directly rostral from, and in close proximity to, the NPO. Despite the lack of co-expression, the nerve fibres projecting onto both the rostral pars distalis (rPD) as well as the large fibre bundles projecting onto the pars intermedia (PI) contain CRH as well as CRH-BP, suggesting that both ACTH release from the rPD as well as the release of PI melanotrope content is regulated via CRH and CRH-BP. Finally, we show via real-time quantitative PCR that expression of hypothalamic CRH and CRH-BP following a 24 h restraint significantly increases, whereas PD CRH-R1 expression decreases; this reflects desensitisation of the PD for hypothalamic CRH output. We conclude that these factors are actively involved in the regulation of acute stress responses in the teleost fish.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0952-5041
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
627-48
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15171705-Amino Acid Sequence, pubmed-meshheading:15171705-Animals, pubmed-meshheading:15171705-Base Sequence, pubmed-meshheading:15171705-Carps, pubmed-meshheading:15171705-Carrier Proteins, pubmed-meshheading:15171705-Corticotropin-Releasing Hormone, pubmed-meshheading:15171705-Humans, pubmed-meshheading:15171705-Hydrocortisone, pubmed-meshheading:15171705-Molecular Sequence Data, pubmed-meshheading:15171705-Phylogeny, pubmed-meshheading:15171705-Pituitary Gland, pubmed-meshheading:15171705-Preoptic Area, pubmed-meshheading:15171705-Protein Isoforms, pubmed-meshheading:15171705-Receptors, Corticotropin-Releasing Hormone, pubmed-meshheading:15171705-Sequence Alignment, pubmed-meshheading:15171705-Sequence Homology, Amino Acid, pubmed-meshheading:15171705-Stress, Psychological
pubmed:year
2004
pubmed:articleTitle
Structural characterisation of a cyprinid (Cyprinus carpio L.) CRH, CRH-BP and CRH-R1, and the role of these proteins in the acute stress response.
pubmed:affiliation
Department of Animal Physiology, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't