Linked Life Data

15158673

Source:http://linkedlifedata.com/resource/pubmed/id/15158673

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-5-25
pubmed:databankReference
pubmed:abstractText
We report the functional characterization of a new UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (ppGalNAc-T) (EC 2.4.1.41) from the human disease-causing parasite, Toxoplasma gondii. This glycosyltransferase is denoted as T. gondii ppGalNAc-T3. These enzymes are responsible for the initial step of mucin-type O-glycosylation: the transfer of GalNAc from the UDP-GalNAc nucleotide sugar donor onto a peptide acceptor. Following an in silico analysis of the publicly available T. gondii DNA database, we used molecular biology approaches to identify and isolate the cDNA encoding this enzyme. The resulting type II membrane protein contains N-terminal cytoplasmic, transmembrane, and C-terminal lumenal domains. Conceptual translation of the cDNA sequence also reveals a stem region and the presence of several important sequence motifs. When the recombinant construct was expressed in stably transfected Drosophila melanogaster S2 cells, the purified protein exhibited glycosyltransferase activity in vitro against glycopeptide, but not "naked" peptide, acceptors. In addition, using reverse transcriptase-PCR, T. gondii ppGalNAc-T3 mRNA was equivalently expressed during the tachyzoite and bradyzoite developmental stages. The identification of T. gondii ppGalNAc-T3 as a functional "follow-up" glycopeptide glycosyltransferase further confirms that this human parasite has its own enzymatic O-glycosylation machinery.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
426
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
231-40
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Functional characterization of a novel Toxoplasma gondii glycosyltransferase: UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T3.
pubmed:affiliation
Department of Biology, University of Pennsylvania, Goddard Laboratories, 415 South University Avenue, Philadelphia, PA 19104, USA.
pubmed:publicationType
Journal Article