Source:http://linkedlifedata.com/resource/pubmed/id/15158467
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-5-25
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| pubmed:abstractText |
Treatment of 3T3-L1 preadipocytes with differentiation inducers triggers a cascade in which C/EBPbeta is rapidly expressed, followed by C/EBPalpha and PPARgamma. C/EBPalpha and PPARgamma then activate the expression of adipocyte genes that produce the differentiated phenotype. Circumstantial evidence indicates that C/EBPbeta activates transcription of the C/EBPalpha and PPARgamma genes, both of which possess C/EBP regulatory elements in their proximal promoters. Although C/EBPbeta is expressed immediately upon induction of differentiation, acquisition of DNA binding activity is delayed for approximately 14h. Chromatin immunoprecipitation (ChIP) analysis conducted 24h after induction revealed that C/EBPbeta binds to C/EBP regulatory elements in the proximal promoters of the C/EBPalpha and PPARgamma genes. After an additional delay ChIP analysis showed that C/EBPalpha binds to its own promoter and to the promoters of the PPARgamma and 422/aP2 genes. These findings support the view that once expressed, C/EBPalpha is responsible for maintaining the expression of PPARgamma and C/EBPalpha, as well as adipocyte proteins (e.g., 422/aP2) in the terminally differentiated state. Together these findings provide compelling evidence that C/EBPbeta, C/EBPalpha, and PPARgamma participate in a cascade during adipogenesis.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0006-291X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
18
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| pubmed:volume |
319
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
235-9
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15158467-3T3-L1 Cells,
pubmed-meshheading:15158467-Adipocytes,
pubmed-meshheading:15158467-Adipose Tissue,
pubmed-meshheading:15158467-Animals,
pubmed-meshheading:15158467-CCAAT-Enhancer-Binding Protein-alpha,
pubmed-meshheading:15158467-CCAAT-Enhancer-Binding Protein-beta,
pubmed-meshheading:15158467-Cell Differentiation,
pubmed-meshheading:15158467-Chromatin,
pubmed-meshheading:15158467-DNA,
pubmed-meshheading:15158467-Immunoblotting,
pubmed-meshheading:15158467-Mice,
pubmed-meshheading:15158467-Phenotype,
pubmed-meshheading:15158467-Precipitin Tests,
pubmed-meshheading:15158467-Promoter Regions, Genetic,
pubmed-meshheading:15158467-Protein Binding,
pubmed-meshheading:15158467-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:15158467-Time Factors,
pubmed-meshheading:15158467-Transcription, Genetic,
pubmed-meshheading:15158467-Transcription Factors
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| pubmed:year |
2004
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| pubmed:articleTitle |
Sequential gene promoter interactions of C/EBPbeta, C/EBPalpha, and PPARgamma during adipogenesis.
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| pubmed:affiliation |
Biological Chemistry, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USA. qtang@jhem.jhmi.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Duplicate Publication
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