Source:http://linkedlifedata.com/resource/pubmed/id/15158465
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-5-25
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| pubmed:abstractText |
Epstein-Barr virus lacking glycoprotein gp85 cannot infect B-cells and epithelial cells. The gp85 belongs to the molecular complex required for virus invasion of B-lymphocyte or epithelial cells. Moreover, there is evidence that gp85 is necessary for virus attachment to epithelial cells. Thirty-six peptides from the entire gp85-sequence were tested in epithelial and lymphoblastoid cell line binding assays to identify gp85-regions involved in virus-cell interaction. Five of these peptides presented high binding activity to Raji, Ramos, P3HR-1, and HeLa cells, but not to erythrocytes; Raji-cell affinity constants were between 80 and 140nM. Of these five peptides, 11435 ((181)TYKRVTEKGDEHVLSLVFGK(200)), 11436 ((201)TKDLPDLRGPFSYPSLTSAQ(220)), and 11438 ((241)YFVPNLKDMFSRAVTMTAAS(260)) bound to a 65kDa protein on Raji-cell surface. These peptides and antibodies induced by them (recognising live EBV-infected cells) inhibited Epstein-Barr virus interaction with cord blood lymphocytes. It is thus probable that gp85-regions defined by peptides 11435, 11436, and 11438 are involved in EBV invasion of B-lymphocytes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-291X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
18
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| pubmed:volume |
319
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
221-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15158465-Animals,
pubmed-meshheading:15158465-B-Lymphocytes,
pubmed-meshheading:15158465-Blotting, Western,
pubmed-meshheading:15158465-Cell Line,
pubmed-meshheading:15158465-Cell Membrane,
pubmed-meshheading:15158465-Cell Separation,
pubmed-meshheading:15158465-Dose-Response Relationship, Drug,
pubmed-meshheading:15158465-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:15158465-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15158465-Epithelial Cells,
pubmed-meshheading:15158465-Flow Cytometry,
pubmed-meshheading:15158465-HeLa Cells,
pubmed-meshheading:15158465-Herpesvirus 4, Human,
pubmed-meshheading:15158465-Humans,
pubmed-meshheading:15158465-Immunoglobulins,
pubmed-meshheading:15158465-Leukocytes,
pubmed-meshheading:15158465-Lymphocytes,
pubmed-meshheading:15158465-Peptides,
pubmed-meshheading:15158465-Polymerase Chain Reaction,
pubmed-meshheading:15158465-Protein Binding,
pubmed-meshheading:15158465-Viral Envelope Proteins
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Identifying gp85-regions involved in Epstein-Barr virus binding to B-lymphocytes.
|
| pubmed:affiliation |
Fundación Instituto de Inmunología de Colombia and Universidad Nacional de Colombia, Bogota, Colombia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|