|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2004-5-25
|
|
pubmed:abstractText |
G protein-coupled receptors (GPCRs) mediate diverse signaling processes, including olfaction. G protein-coupled receptor kinases (GRKs) are important regulators of G protein signal transduction that specifically phosphorylate activated GPCRs to terminate signaling. Despite previously described roles for GRKs in GPCR signal downregulation, animals lacking C. elegans G protein-coupled receptor kinase-2 (Ce-grk-2) function are not hypersensitive to odorants. Instead, decreased Ce-grk-2 function in adult sensory neurons profoundly disrupts chemosensation, based on both behavioral analysis and Ca(2+) imaging. Although mammalian arrestin proteins cooperate with GRKs in receptor desensitization, loss of C. elegans arrestin-1 (arr-1) does not disrupt chemosensation. Either overexpression of the C. elegans Galpha subunit odr-3 or loss of eat-16, which encodes a regulator of G protein signaling (RGS) protein, restores chemosensation in Ce-grk-2 mutants. These results demonstrate that loss of GRK function can lead to reduced GPCR signal transduction and suggest an important role for RGS proteins in the regulation of chemosensation.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arrestins,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/EAT-16 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein Regulators,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Adrenergic Receptor Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/odr-3 protein, C elegans
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0896-6273
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
27
|
|
pubmed:volume |
42
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
581-93
|
|
pubmed:dateRevised |
2011-5-23
|
|
pubmed:meshHeading |
pubmed-meshheading:15157420-Animals,
pubmed-meshheading:15157420-Arrestins,
pubmed-meshheading:15157420-Caenorhabditis elegans,
pubmed-meshheading:15157420-Caenorhabditis elegans Proteins,
pubmed-meshheading:15157420-Chemoreceptor Cells,
pubmed-meshheading:15157420-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:15157420-GTP-Binding Protein Regulators,
pubmed-meshheading:15157420-GTP-Binding Protein alpha Subunits, Gi-Go,
pubmed-meshheading:15157420-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:15157420-Mutation,
pubmed-meshheading:15157420-Nervous System,
pubmed-meshheading:15157420-Neurons, Afferent,
pubmed-meshheading:15157420-Phosphoproteins,
pubmed-meshheading:15157420-Phosphotransferases,
pubmed-meshheading:15157420-Receptors, G-Protein-Coupled,
pubmed-meshheading:15157420-Signal Transduction,
pubmed-meshheading:15157420-beta-Adrenergic Receptor Kinases
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
G protein-coupled receptor kinase function is essential for chemosensation in C. elegans.
|
|
pubmed:affiliation |
Department of Biology, University of California, San Diego, La Jolla, CA 92093 USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
