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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1992-10-7
|
| pubmed:abstractText |
The effect of commonly used food antioxidants on recombinant tumor necrosis factor alpha (rTNF-alpha)-induced cytotoxicity, growth enhancement and adhesion has been evaluated. Butylated hydroxyanisole (BHA) and 4-hydroxymethyl-2,6-di-t-butylphenol (HBP) were the only two of nine antioxidants that completely inhibited rTNF-alpha-induced cytotoxicity in L929 and WEHI 164 fibrosarcoma cells. Ethoxyquin, propyl gallate and butylated hydroquinone only partially inhibited rTNF-alpha-induced cytotoxicity, while the antioxidants butylated hydroxytoluene (BHT), alpha-tocopherol, ascorbic acid and thiodipropionic acid had minimal effects. The only difference between the molecular structure of the efficient HBP and the non-efficient BHT, is a hydroxymethyl group instead of a hydroxyl group on the phenolic ring. Neither BHA nor BHT inhibited the activation of NF kappa B after 10 or 60 min challenge with rTNF-alpha in L929 cells. BHA also inhibited rTNF-alpha-induced, but not rIL-1 beta-induced growth enhancement in FS-4 fibroblasts. Further, BHA blocked both rTNF-alpha-induced and rIL-1 beta-induced prostaglandin E2 synthesis in FS-4 fibroblasts. BHA inhibited the rTNF-alpha-induced release of arachidonic acid in both FS-4 and L929 cells, suggesting that BHA inhibits cellular phospholipase(s). Neither alpha-tocopherol nor BHA inhibited rTNF-alpha-induced adhesiveness of human endothelial cells. The results indicate that BHA is a specific and potent inhibitor of rTNF-alpha- and rTNF-beta-induced cytotoxicity, as well as of rTNF-alpha-induced growth enhancement.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Butylated Hydroxyanisole,
http://linkedlifedata.com/resource/pubmed/chemical/Butylated Hydroxytoluene,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1043-4666
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
269-80
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1515551-Animals,
pubmed-meshheading:1515551-Antioxidants,
pubmed-meshheading:1515551-Arachidonic Acid,
pubmed-meshheading:1515551-Base Sequence,
pubmed-meshheading:1515551-Butylated Hydroxyanisole,
pubmed-meshheading:1515551-Butylated Hydroxytoluene,
pubmed-meshheading:1515551-Cell Adhesion,
pubmed-meshheading:1515551-Cell Division,
pubmed-meshheading:1515551-Cytotoxicity, Immunologic,
pubmed-meshheading:1515551-Dinoprostone,
pubmed-meshheading:1515551-Endothelium, Vascular,
pubmed-meshheading:1515551-Fibroblasts,
pubmed-meshheading:1515551-Humans,
pubmed-meshheading:1515551-Interleukin-1,
pubmed-meshheading:1515551-Mice,
pubmed-meshheading:1515551-Molecular Sequence Data,
pubmed-meshheading:1515551-NF-kappa B,
pubmed-meshheading:1515551-Tumor Cells, Cultured,
pubmed-meshheading:1515551-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Butylated hydroxyanisole specifically inhibits tumor necrosis factor-induced cytotoxicity and growth enhancement.
|
| pubmed:affiliation |
Department of Clinical Chemistry, Trondheim Regional Hospital, University of Norway.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|