Linked Life Data

1515415

Source:http://linkedlifedata.com/resource/pubmed/id/1515415

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-10-2
pubmed:abstractText
The c-ets1 gene product (Ets1) is the prototype of a family of sequence-specific transcriptional activators which have been implicated in various developmental processes and in the response of cells to a variety of extracellular stimuli. We report here a structure-function analysis of the DNA binding and transcriptional activation properties of Ets1. The minimal region required for specific DNA binding is located at the carboxy-terminus of Ets1, a domain highly conserved in all known members of the Ets family. Transcriptional activation by Ets1 in mammalian cells requires an additional domain of 110 amino acids characterized by a high content of acidic residues and localized in the amino-terminal half of the protein. This domain also functions as a transcriptional activation domain in yeast cells when linked to the heterologous DNA binding domain of Gal4. In contrast to its conservation in Ets1 proteins across vertebrate species, this activation domain is not conserved in other members of the Ets family. These results indicate that an important level of specificity between different members of the Ets family may reside in the differential interactions of their respective activation domains with distinct general transcription factors or different associated coactivators.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1043-4674
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:geneSymbol
c-ets1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
512-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Analysis of the DNA binding and transcriptional activation properties of the Ets1 oncoprotein.
pubmed:affiliation
Laboratoire d'Oncologie Virale et Cellulaire, CNRS URA 1443, Centre Universitaire, Orsay, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't