Linked Life Data

15151926

Source:http://linkedlifedata.com/resource/pubmed/id/15151926

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-9-21
pubmed:abstractText
Roe deer are seasonal breeders and show cyclic variation in testicular volume and cellular differentiation within the tubular and interstitial testis compartment. We have employed the roe deer as a model to elucidate the expression of the postpubertal Leydig cell marker INSL3 during seasonal changes in Leydig cell differentiation. Roe deer testis and serum samples were collected bimonthly throughout the complete reproductive cycle. Peak levels of testicular Insl3 mRNA and INSL3 immunoprotein were detected well before the onset of rut in April and coincided with the highest percentage of INSL3-positive cell number/square millimeter of testicular interstitial area. During the winter (December, February), roe deer INSL3 was exclusively detected in a subpopulation of alpha-actin-negative, spindle-shaped peritubular cells. Concordant with the increase in INSL3 production in April and 1 mo after the reported LH peak, a sharp increase in serum testosterone concentrations was observed. High serum testosterone concentrations coincided with the presence of detectable 17alpha-hydroxylase, mRNA and protein, in Leydig cells. Upregulation of INSL3 production in spring appeared to reflect LH-dependent differentiation of Leydig cells. The considerable changes in percentage of INSL3 immunopositive cells within the numerically constant interstitial cell population indicated cyclic seasonal de- and redifferentiation of Leydig cells. A complex functional role of the INSL3/LGR8 ligand-receptor system in the roe deer testis was suggested by the detection of specific hybridization signals for roe deer Lgr8 transcripts in Sertoli cells of the roe deer testis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1079-87
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15151926-Animals, pubmed-meshheading:15151926-Cell Differentiation, pubmed-meshheading:15151926-Deer, pubmed-meshheading:15151926-Gene Expression Regulation, pubmed-meshheading:15151926-Hormones, pubmed-meshheading:15151926-Insulin, pubmed-meshheading:15151926-Leydig Cells, pubmed-meshheading:15151926-Luteinizing Hormone, pubmed-meshheading:15151926-Male, pubmed-meshheading:15151926-Proteins, pubmed-meshheading:15151926-RNA, Messenger, pubmed-meshheading:15151926-Receptors, G-Protein-Coupled, pubmed-meshheading:15151926-Receptors, Peptide, pubmed-meshheading:15151926-Reproduction, pubmed-meshheading:15151926-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15151926-Seasons, pubmed-meshheading:15151926-Sertoli Cells, pubmed-meshheading:15151926-Testis, pubmed-meshheading:15151926-Testosterone
pubmed:year
2004
pubmed:articleTitle
Seasonal expression of INSL3 and Lgr8/Insl3 receptor transcripts indicates variable differentiation of Leydig cells in the roe deer testis.
pubmed:affiliation
Department of Human Anatomy and Cell Science, Faculty of Medicine, University of Mannitoba, Winnipeg, MB, Canada. hombach@cc.manitoba.ca
pubmed:publicationType
Journal Article, Comparative Study